In vitro and in vivo pro-angiogenic effects of thymosin-β4-derived peptides

Monica Dettin, Francesca Ghezzo, Maria Teresa Conconi, Luca Urbani, Gabriella D'Auria, Lucia Falcigno, Diego Guidolin, Beatrice Nico, Domenico Ribatti, Carlo Di Bello, Pier Paolo Parnigotto

Research output: Contribution to journalArticlepeer-review

Abstract

Thymosin-β4 (Tβ4) is a G-actin sequestering peptide involved in regeneration and remodeling of injured tissues. In this work, we have designed and synthesized three peptide sequences containing the N-terminus (TYB4-n), the central part (TYB4-i) or the C-terminus (TYB4-c) of Tβ4. All fragments are overlapping on the main central binding actin site. After a structural characterization, we have evaluated in vitro and in vivo their pro-angiogenic effects. The results of this study have shown that: (i) each fragment reproduces the native conformation; (ii) Tβ4-derived peptides exert both in vitro and in vivo pro-angiogenic effects; (iii) their in vitro effect seem to be related to the activation of several signaling pathways and is positively modulated by the N-terminus of Tβ4.

Original languageEnglish
Pages (from-to)299-307
Number of pages9
JournalCellular Immunology
Volume271
Issue number2
DOIs
Publication statusPublished - 2011

Keywords

  • Angiogenesis
  • Chorioallantoic membrane
  • Conformational studies
  • HUVEC
  • Peptides
  • Thymosin-β4

ASJC Scopus subject areas

  • Immunology

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