Abstract
Interdental papilla are an interesting source of mesenchymal stromal cells (GinPaMSCs), which are easy to isolate and expand in vitro. In our laboratory, GinPaMSCs were isolated, expanded, and characterized by studying their secretome before and after priming with paclitaxel (PTX). The secretome of GinPaMSCs did not affect the growth of cancer cell lines tested in vitro, whereas the secretome of GinPaMSCs primed with paclitaxel (GinPaMSCs/PTX) exerted a significant anticancer effect. GinPaMSCs were able to uptake and then release paclitaxel in amounts pharmacologically effective against cancer cells, as demonstrated in vitro by the direct activity of GinPaMSCs/PTX and their secretome against both human pancreatic carcinoma and squamous carcinoma cells. PTX was associated with extracellular vesicles (EVs) secreted by cells (EVs/PTX), suggesting that PTX is incorporated into exosomes during their biogenesis. The isolation of mesenchymal stromal cells (MSCs) from gingiva is less invasive than that from other tissues (such as bone marrow and fat), and GinPaMSCs provide an optimal substrate for drug-priming to obtain EVs/PTX having anticancer activity. This research may contribute to develop new strategies of cell-mediated drug delivery by EVs that are easy to store without losing function, and could have a superior safety profile in therapy.
| Original language | English |
|---|---|
| Article number | 61 |
| Journal | Pharmaceutics |
| Volume | 11 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 1 2019 |
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Keywords
- Drug delivery
- Exosomes
- Gingiva mesenchymal stromal cells
- Paclitaxel
- Squamous cell carcinoma
ASJC Scopus subject areas
- Pharmaceutical Science
Cite this
In vitro anticancer activity of extracellular vesicles (evs) secreted by gingival mesenchymal stromal cells primed with paclitaxel. / Coccè, Valentina; Franzè, Silvia; Brini, Anna Teresa; Giannì, Aldo Bruno; Pascucci, Luisa; Ciusani, Emilio; Alessandri, Giulio; Farronato, Giampietro; Cavicchini, Loredana; Sordi, Valeria; Paroni, Rita; Cas, Michele Dei; Cilurzo, Francesco; Pessina, Augusto.
In: Pharmaceutics, Vol. 11, No. 2, 61, 01.02.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro anticancer activity of extracellular vesicles (evs) secreted by gingival mesenchymal stromal cells primed with paclitaxel
AU - Coccè, Valentina
AU - Franzè, Silvia
AU - Brini, Anna Teresa
AU - Giannì, Aldo Bruno
AU - Pascucci, Luisa
AU - Ciusani, Emilio
AU - Alessandri, Giulio
AU - Farronato, Giampietro
AU - Cavicchini, Loredana
AU - Sordi, Valeria
AU - Paroni, Rita
AU - Cas, Michele Dei
AU - Cilurzo, Francesco
AU - Pessina, Augusto
PY - 2019/2/1
Y1 - 2019/2/1
N2 - Interdental papilla are an interesting source of mesenchymal stromal cells (GinPaMSCs), which are easy to isolate and expand in vitro. In our laboratory, GinPaMSCs were isolated, expanded, and characterized by studying their secretome before and after priming with paclitaxel (PTX). The secretome of GinPaMSCs did not affect the growth of cancer cell lines tested in vitro, whereas the secretome of GinPaMSCs primed with paclitaxel (GinPaMSCs/PTX) exerted a significant anticancer effect. GinPaMSCs were able to uptake and then release paclitaxel in amounts pharmacologically effective against cancer cells, as demonstrated in vitro by the direct activity of GinPaMSCs/PTX and their secretome against both human pancreatic carcinoma and squamous carcinoma cells. PTX was associated with extracellular vesicles (EVs) secreted by cells (EVs/PTX), suggesting that PTX is incorporated into exosomes during their biogenesis. The isolation of mesenchymal stromal cells (MSCs) from gingiva is less invasive than that from other tissues (such as bone marrow and fat), and GinPaMSCs provide an optimal substrate for drug-priming to obtain EVs/PTX having anticancer activity. This research may contribute to develop new strategies of cell-mediated drug delivery by EVs that are easy to store without losing function, and could have a superior safety profile in therapy.
AB - Interdental papilla are an interesting source of mesenchymal stromal cells (GinPaMSCs), which are easy to isolate and expand in vitro. In our laboratory, GinPaMSCs were isolated, expanded, and characterized by studying their secretome before and after priming with paclitaxel (PTX). The secretome of GinPaMSCs did not affect the growth of cancer cell lines tested in vitro, whereas the secretome of GinPaMSCs primed with paclitaxel (GinPaMSCs/PTX) exerted a significant anticancer effect. GinPaMSCs were able to uptake and then release paclitaxel in amounts pharmacologically effective against cancer cells, as demonstrated in vitro by the direct activity of GinPaMSCs/PTX and their secretome against both human pancreatic carcinoma and squamous carcinoma cells. PTX was associated with extracellular vesicles (EVs) secreted by cells (EVs/PTX), suggesting that PTX is incorporated into exosomes during their biogenesis. The isolation of mesenchymal stromal cells (MSCs) from gingiva is less invasive than that from other tissues (such as bone marrow and fat), and GinPaMSCs provide an optimal substrate for drug-priming to obtain EVs/PTX having anticancer activity. This research may contribute to develop new strategies of cell-mediated drug delivery by EVs that are easy to store without losing function, and could have a superior safety profile in therapy.
KW - Drug delivery
KW - Exosomes
KW - Gingiva mesenchymal stromal cells
KW - Paclitaxel
KW - Squamous cell carcinoma
UR - http://www.scopus.com/inward/record.url?scp=85063158115&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85063158115&partnerID=8YFLogxK
U2 - 10.3390/pharmaceutics11020061
DO - 10.3390/pharmaceutics11020061
M3 - Article
AN - SCOPUS:85063158115
VL - 11
JO - Pharmaceutics
JF - Pharmaceutics
SN - 1999-4923
IS - 2
M1 - 61
ER -