Abstract
Aims: This study compared in vitro activities of Actichelated® propolis (a multicomposite material obtained with mechano-chemichal activation) and of a hydroalcoholic extract of propolis. Methods and Results: Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC), determined by means of microdilution broth method, against five strains of Staphylococcus aureus, Streptococcus pyogenes, Haemophilus influenzae, Enterococcus spp., Escherichia coli, Proteus mirabilis and Pseudomonas aeruginosa, showed a greater potency of Actichelated® propolis (MIC range: 0.016-4 mg flavonoids ml -1) in respect to the hydroalcoholic extract (MIC range: 0.08-21.4 mg flavonoids ml-1). Concentrations of Actichelated® propolis active against adenovirus, influenza virus, parainfluenza virus and herpes virus type 1 were at least 10 times lower than those of the hydroalcoholic extract. Preincubation of Strep. pyogenes and H. influenzae with subinhibitory concentrations of Actichelated® propolis (1/4 and 1/8 x MIC) significantly reduced the number of bacteria that adhered to human buccal cells. Conclusions: Actichelated® propolis has proven to possess antibacterial and antiviral activity higher than a hydroalcoholic extract, being also able to interfere on bacterial adhesion to human oral cells. Significance and Impact of the Study: This new formulation of propolis showing better antimicrobial and physical characteristics could improve the application of propolis in respiratory tract infections.
| Original language | English |
|---|---|
| Pages (from-to) | 1914-1921 |
| Number of pages | 8 |
| Journal | Journal of Applied Microbiology |
| Volume | 103 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Nov 2007 |
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Keywords
- Actichelated propolis
- Antibacterial activity
- Antiviral activity
- Bacterial adhesion
- Mechano-chemical activation
- Propolis
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biotechnology
- Applied Microbiology and Biotechnology
- Microbiology
Cite this
In vitro antimicrobial activity of a novel propolis formulation (Actichelated propolis). / Drago, L.; De Vecchi, E.; Nicola, L.; Gismondo, M. R.
In: Journal of Applied Microbiology, Vol. 103, No. 5, 11.2007, p. 1914-1921.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro antimicrobial activity of a novel propolis formulation (Actichelated propolis)
AU - Drago, L.
AU - De Vecchi, E.
AU - Nicola, L.
AU - Gismondo, M. R.
PY - 2007/11
Y1 - 2007/11
N2 - Aims: This study compared in vitro activities of Actichelated® propolis (a multicomposite material obtained with mechano-chemichal activation) and of a hydroalcoholic extract of propolis. Methods and Results: Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC), determined by means of microdilution broth method, against five strains of Staphylococcus aureus, Streptococcus pyogenes, Haemophilus influenzae, Enterococcus spp., Escherichia coli, Proteus mirabilis and Pseudomonas aeruginosa, showed a greater potency of Actichelated® propolis (MIC range: 0.016-4 mg flavonoids ml -1) in respect to the hydroalcoholic extract (MIC range: 0.08-21.4 mg flavonoids ml-1). Concentrations of Actichelated® propolis active against adenovirus, influenza virus, parainfluenza virus and herpes virus type 1 were at least 10 times lower than those of the hydroalcoholic extract. Preincubation of Strep. pyogenes and H. influenzae with subinhibitory concentrations of Actichelated® propolis (1/4 and 1/8 x MIC) significantly reduced the number of bacteria that adhered to human buccal cells. Conclusions: Actichelated® propolis has proven to possess antibacterial and antiviral activity higher than a hydroalcoholic extract, being also able to interfere on bacterial adhesion to human oral cells. Significance and Impact of the Study: This new formulation of propolis showing better antimicrobial and physical characteristics could improve the application of propolis in respiratory tract infections.
AB - Aims: This study compared in vitro activities of Actichelated® propolis (a multicomposite material obtained with mechano-chemichal activation) and of a hydroalcoholic extract of propolis. Methods and Results: Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC), determined by means of microdilution broth method, against five strains of Staphylococcus aureus, Streptococcus pyogenes, Haemophilus influenzae, Enterococcus spp., Escherichia coli, Proteus mirabilis and Pseudomonas aeruginosa, showed a greater potency of Actichelated® propolis (MIC range: 0.016-4 mg flavonoids ml -1) in respect to the hydroalcoholic extract (MIC range: 0.08-21.4 mg flavonoids ml-1). Concentrations of Actichelated® propolis active against adenovirus, influenza virus, parainfluenza virus and herpes virus type 1 were at least 10 times lower than those of the hydroalcoholic extract. Preincubation of Strep. pyogenes and H. influenzae with subinhibitory concentrations of Actichelated® propolis (1/4 and 1/8 x MIC) significantly reduced the number of bacteria that adhered to human buccal cells. Conclusions: Actichelated® propolis has proven to possess antibacterial and antiviral activity higher than a hydroalcoholic extract, being also able to interfere on bacterial adhesion to human oral cells. Significance and Impact of the Study: This new formulation of propolis showing better antimicrobial and physical characteristics could improve the application of propolis in respiratory tract infections.
KW - Actichelated propolis
KW - Antibacterial activity
KW - Antiviral activity
KW - Bacterial adhesion
KW - Mechano-chemical activation
KW - Propolis
UR - http://www.scopus.com/inward/record.url?scp=35448930797&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=35448930797&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2672.2007.03421.x
DO - 10.1111/j.1365-2672.2007.03421.x
M3 - Article
C2 - 17953601
AN - SCOPUS:35448930797
VL - 103
SP - 1914
EP - 1921
JO - Journal of Applied Microbiology
JF - Journal of Applied Microbiology
SN - 1364-5072
IS - 5
ER -