In vitro antimicrobial activity of chlorquinaldol against microorganisms responsible for skin and soft tissue infections: Comparative evaluation with gentamicin and fusidic acid

Monica Bortolin, Alessandro Bidossi, Elena De Vecchi, Maura Avveniente, Lorenzo Drago

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Skin and soft tissue infections (SSTIs) are a major therapeutic challenge for clinicians. The emergence of pathogens with decreased susceptibility to available therapies has become an emerging problem often associated with treatment failure. Hence, there is an urgent need for novel broad-spectrum antimicrobial agents. The purpose of this study was to assess the feasibility of chlorquinaldol as an alternative approach to currently used topical antibiotics for the treatment of skin and soft tissue infections. The activity of chlorquinaldol was investigated against a collection of bacterial isolates responsible for skin infections, including strains resistant to fusidic acid and gentamicin. After determination of MIC and MBC, time-kill experiments were carried out by counting colonies grown after 0, 3, 6, 9, 24, and 48 h of incubation with concentrations equal to 1/4×, 1/2×, 1×, 2×, and 4× MIC of chlorquinaldol, gentamicin, or fusidic acid. Staphylococci resulted the Gram-positives most sensitive to chlorquinaldol, with MIC-values ranging from 0.016 to 0.5 mg/L. A lower activity was observed against Gram-negative bacteria, with 77% of the isolates being inhibited at concentrations ranging from 128 to 512 mg/L. Generally, in time-kill studies, chlorquinaldol showed a bactericidal activity at the higher concentrations (2×, 4× MIC) after 24-48 h of incubation. In conclusion, chlorquinaldol may represent a valuable alternative to conventional topical antibiotics for the treatment of skin and soft tissue infections.

Original languageEnglish
Article number1039
JournalFrontiers in Microbiology
Volume8
Issue numberJUN
DOIs
Publication statusPublished - Jun 8 2017

Fingerprint

Chlorquinaldol
Fusidic Acid
Soft Tissue Infections
Gentamicins
Skin
Anti-Bacterial Agents
Anti-Infective Agents
Gram-Negative Bacteria
Treatment Failure
Staphylococcus
In Vitro Techniques

Keywords

  • Chlorquinaldol
  • Fusidic acid
  • Gentamicin
  • Minimum bactericidal concentration
  • Minimum inhibitory concentration
  • Skin infections
  • Time-kill curves

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

@article{2b1b0989efc2494b9eeb562e5b0a8ced,
title = "In vitro antimicrobial activity of chlorquinaldol against microorganisms responsible for skin and soft tissue infections: Comparative evaluation with gentamicin and fusidic acid",
abstract = "Skin and soft tissue infections (SSTIs) are a major therapeutic challenge for clinicians. The emergence of pathogens with decreased susceptibility to available therapies has become an emerging problem often associated with treatment failure. Hence, there is an urgent need for novel broad-spectrum antimicrobial agents. The purpose of this study was to assess the feasibility of chlorquinaldol as an alternative approach to currently used topical antibiotics for the treatment of skin and soft tissue infections. The activity of chlorquinaldol was investigated against a collection of bacterial isolates responsible for skin infections, including strains resistant to fusidic acid and gentamicin. After determination of MIC and MBC, time-kill experiments were carried out by counting colonies grown after 0, 3, 6, 9, 24, and 48 h of incubation with concentrations equal to 1/4×, 1/2×, 1×, 2×, and 4× MIC of chlorquinaldol, gentamicin, or fusidic acid. Staphylococci resulted the Gram-positives most sensitive to chlorquinaldol, with MIC-values ranging from 0.016 to 0.5 mg/L. A lower activity was observed against Gram-negative bacteria, with 77{\%} of the isolates being inhibited at concentrations ranging from 128 to 512 mg/L. Generally, in time-kill studies, chlorquinaldol showed a bactericidal activity at the higher concentrations (2×, 4× MIC) after 24-48 h of incubation. In conclusion, chlorquinaldol may represent a valuable alternative to conventional topical antibiotics for the treatment of skin and soft tissue infections.",
keywords = "Chlorquinaldol, Fusidic acid, Gentamicin, Minimum bactericidal concentration, Minimum inhibitory concentration, Skin infections, Time-kill curves",
author = "Monica Bortolin and Alessandro Bidossi and Vecchi, {Elena De} and Maura Avveniente and Lorenzo Drago",
year = "2017",
month = "6",
day = "8",
doi = "10.3389/fmicb.2017.01039",
language = "English",
volume = "8",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S. A.",
number = "JUN",

}

TY - JOUR

T1 - In vitro antimicrobial activity of chlorquinaldol against microorganisms responsible for skin and soft tissue infections

T2 - Comparative evaluation with gentamicin and fusidic acid

AU - Bortolin, Monica

AU - Bidossi, Alessandro

AU - Vecchi, Elena De

AU - Avveniente, Maura

AU - Drago, Lorenzo

PY - 2017/6/8

Y1 - 2017/6/8

N2 - Skin and soft tissue infections (SSTIs) are a major therapeutic challenge for clinicians. The emergence of pathogens with decreased susceptibility to available therapies has become an emerging problem often associated with treatment failure. Hence, there is an urgent need for novel broad-spectrum antimicrobial agents. The purpose of this study was to assess the feasibility of chlorquinaldol as an alternative approach to currently used topical antibiotics for the treatment of skin and soft tissue infections. The activity of chlorquinaldol was investigated against a collection of bacterial isolates responsible for skin infections, including strains resistant to fusidic acid and gentamicin. After determination of MIC and MBC, time-kill experiments were carried out by counting colonies grown after 0, 3, 6, 9, 24, and 48 h of incubation with concentrations equal to 1/4×, 1/2×, 1×, 2×, and 4× MIC of chlorquinaldol, gentamicin, or fusidic acid. Staphylococci resulted the Gram-positives most sensitive to chlorquinaldol, with MIC-values ranging from 0.016 to 0.5 mg/L. A lower activity was observed against Gram-negative bacteria, with 77% of the isolates being inhibited at concentrations ranging from 128 to 512 mg/L. Generally, in time-kill studies, chlorquinaldol showed a bactericidal activity at the higher concentrations (2×, 4× MIC) after 24-48 h of incubation. In conclusion, chlorquinaldol may represent a valuable alternative to conventional topical antibiotics for the treatment of skin and soft tissue infections.

AB - Skin and soft tissue infections (SSTIs) are a major therapeutic challenge for clinicians. The emergence of pathogens with decreased susceptibility to available therapies has become an emerging problem often associated with treatment failure. Hence, there is an urgent need for novel broad-spectrum antimicrobial agents. The purpose of this study was to assess the feasibility of chlorquinaldol as an alternative approach to currently used topical antibiotics for the treatment of skin and soft tissue infections. The activity of chlorquinaldol was investigated against a collection of bacterial isolates responsible for skin infections, including strains resistant to fusidic acid and gentamicin. After determination of MIC and MBC, time-kill experiments were carried out by counting colonies grown after 0, 3, 6, 9, 24, and 48 h of incubation with concentrations equal to 1/4×, 1/2×, 1×, 2×, and 4× MIC of chlorquinaldol, gentamicin, or fusidic acid. Staphylococci resulted the Gram-positives most sensitive to chlorquinaldol, with MIC-values ranging from 0.016 to 0.5 mg/L. A lower activity was observed against Gram-negative bacteria, with 77% of the isolates being inhibited at concentrations ranging from 128 to 512 mg/L. Generally, in time-kill studies, chlorquinaldol showed a bactericidal activity at the higher concentrations (2×, 4× MIC) after 24-48 h of incubation. In conclusion, chlorquinaldol may represent a valuable alternative to conventional topical antibiotics for the treatment of skin and soft tissue infections.

KW - Chlorquinaldol

KW - Fusidic acid

KW - Gentamicin

KW - Minimum bactericidal concentration

KW - Minimum inhibitory concentration

KW - Skin infections

KW - Time-kill curves

UR - http://www.scopus.com/inward/record.url?scp=85020437905&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020437905&partnerID=8YFLogxK

U2 - 10.3389/fmicb.2017.01039

DO - 10.3389/fmicb.2017.01039

M3 - Article

AN - SCOPUS:85020437905

VL - 8

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

IS - JUN

M1 - 1039

ER -