Abstract
Three commercial samples consisting of enriched calcium-free caseinophosphopeptides (CPP), enriched calcium-bound caseinophosphopeptides (Ca-CPP) and an enzymatically hydrolyzed casein (hCN) were in vitro digested according to COST-Infogest protocol. As assessed by UPLC-HR-MS/MS, the digests contained 207–235 unique caseinophosphopeptides, and the species presenting the cluster sssEE were more abundant in CPP digest. The antioxidant activity at three different doses of each digest was firstly evaluated on human intestinal Caco-2/HT-29 70/30 co-culture. In presence of AAPH, hCN and CPP digests displayed a dose-dependent antioxidant activity equal or even greater than Vitamin C. In presence of Fe2+, the digests exerted an antioxidant activity mainly at the highest dose. Antioxidant activities of the intestinal metabolized digests was then evaluated on human osteoblast (Saos-2) cells. The digests exerted an antioxidant activity in presence of AAPH, but not in presence of Fe2+. These results highlight milk-derived peptides as potential dietary supplements for gut and bone health.
| Original language | English |
|---|---|
| Article number | 103673 |
| Journal | Journal of Functional Foods |
| DOIs | |
| Publication status | Accepted/In press - Jan 1 2019 |
Keywords
- Antioxidant activity
- Caco-2/HT-29 co-culture
- Caseinophosphopeptides
- Gastrointestinal digestion
- Osteoblast-like cells Saos-2
- ROS
ASJC Scopus subject areas
- Food Science
- Medicine (miscellaneous)
- Nutrition and Dietetics
Cite this
In vitro antioxidant properties of digests of hydrolyzed casein and caseinophosphopeptide preparations in cell models of human intestine and osteoblasts. / Bottani, Michela; Cattaneo, Stefano; Pica, Valentina; Stuknytė, Milda; De Noni, Ivano; Ferraretto, Anita.
In: Journal of Functional Foods, 01.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro antioxidant properties of digests of hydrolyzed casein and caseinophosphopeptide preparations in cell models of human intestine and osteoblasts
AU - Bottani, Michela
AU - Cattaneo, Stefano
AU - Pica, Valentina
AU - Stuknytė, Milda
AU - De Noni, Ivano
AU - Ferraretto, Anita
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Three commercial samples consisting of enriched calcium-free caseinophosphopeptides (CPP), enriched calcium-bound caseinophosphopeptides (Ca-CPP) and an enzymatically hydrolyzed casein (hCN) were in vitro digested according to COST-Infogest protocol. As assessed by UPLC-HR-MS/MS, the digests contained 207–235 unique caseinophosphopeptides, and the species presenting the cluster sssEE were more abundant in CPP digest. The antioxidant activity at three different doses of each digest was firstly evaluated on human intestinal Caco-2/HT-29 70/30 co-culture. In presence of AAPH, hCN and CPP digests displayed a dose-dependent antioxidant activity equal or even greater than Vitamin C. In presence of Fe2+, the digests exerted an antioxidant activity mainly at the highest dose. Antioxidant activities of the intestinal metabolized digests was then evaluated on human osteoblast (Saos-2) cells. The digests exerted an antioxidant activity in presence of AAPH, but not in presence of Fe2+. These results highlight milk-derived peptides as potential dietary supplements for gut and bone health.
AB - Three commercial samples consisting of enriched calcium-free caseinophosphopeptides (CPP), enriched calcium-bound caseinophosphopeptides (Ca-CPP) and an enzymatically hydrolyzed casein (hCN) were in vitro digested according to COST-Infogest protocol. As assessed by UPLC-HR-MS/MS, the digests contained 207–235 unique caseinophosphopeptides, and the species presenting the cluster sssEE were more abundant in CPP digest. The antioxidant activity at three different doses of each digest was firstly evaluated on human intestinal Caco-2/HT-29 70/30 co-culture. In presence of AAPH, hCN and CPP digests displayed a dose-dependent antioxidant activity equal or even greater than Vitamin C. In presence of Fe2+, the digests exerted an antioxidant activity mainly at the highest dose. Antioxidant activities of the intestinal metabolized digests was then evaluated on human osteoblast (Saos-2) cells. The digests exerted an antioxidant activity in presence of AAPH, but not in presence of Fe2+. These results highlight milk-derived peptides as potential dietary supplements for gut and bone health.
KW - Antioxidant activity
KW - Caco-2/HT-29 co-culture
KW - Caseinophosphopeptides
KW - Gastrointestinal digestion
KW - Osteoblast-like cells Saos-2
KW - ROS
UR - http://www.scopus.com/inward/record.url?scp=85076117541&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85076117541&partnerID=8YFLogxK
U2 - 10.1016/j.jff.2019.103673
DO - 10.1016/j.jff.2019.103673
M3 - Article
AN - SCOPUS:85076117541
JO - Journal of Functional Foods
JF - Journal of Functional Foods
SN - 1756-4646
M1 - 103673
ER -