In vitro assessment of phagocytosis of bovine collagen by human monocytes/macrophages using a spectrophotometric method

G. Ciapetti, E. Verri, D. Granchi, E. Cenni, S. Gamberini, D. Benetti, M. Mian, A. Pizzoferrato

Research output: Contribution to journalArticle

Abstract

The use of a wound dressing with covering and haemostatic properties significantly improves wound healing. In this study, a lyophilized bovine collagen sponge used for the treatment of wounds and ulcerae has been tested in a cell culture system. Phagocytosis of collagen fragments by human blood monocytes/macrophages has been investigated. For the assessment of collagen ingestion by mononuclear phagocytes, a picrosirius dye specific for collagen molecules has been used. By adapting this histochemical technique to microplate cell culture system, replicate monocyte cultures are assayed. Collagen content is determined by evaluating spectrophotometrically at 540 nm the absorbance of a sirius red/picric acid solution. Using this simple and sensitive method, the phagocytosis of bovine collagen by LPS-stimulated monocytes/macrophages has been ascertained.

Original languageEnglish
Pages (from-to)1703-1707
Number of pages5
JournalBiomaterials
Volume17
Issue number17
DOIs
Publication statusPublished - Sep 1996

Fingerprint

Macrophages
Phagocytosis
Collagen
Monocytes
Cell culture
Cell Culture Techniques
Wounds and Injuries
Porifera
Hemostatics
Bandages
Phagocytes
Wound Healing
In Vitro Techniques
Blood
Coloring Agents
Dyes
Eating
Molecules
Acids

Keywords

  • Collagen
  • Monocytes
  • Phagocytosis
  • Picrosirius

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

In vitro assessment of phagocytosis of bovine collagen by human monocytes/macrophages using a spectrophotometric method. / Ciapetti, G.; Verri, E.; Granchi, D.; Cenni, E.; Gamberini, S.; Benetti, D.; Mian, M.; Pizzoferrato, A.

In: Biomaterials, Vol. 17, No. 17, 09.1996, p. 1703-1707.

Research output: Contribution to journalArticle

Ciapetti, G. ; Verri, E. ; Granchi, D. ; Cenni, E. ; Gamberini, S. ; Benetti, D. ; Mian, M. ; Pizzoferrato, A. / In vitro assessment of phagocytosis of bovine collagen by human monocytes/macrophages using a spectrophotometric method. In: Biomaterials. 1996 ; Vol. 17, No. 17. pp. 1703-1707.
@article{12de6973c74d4487a51701bc5a9899c8,
title = "In vitro assessment of phagocytosis of bovine collagen by human monocytes/macrophages using a spectrophotometric method",
abstract = "The use of a wound dressing with covering and haemostatic properties significantly improves wound healing. In this study, a lyophilized bovine collagen sponge used for the treatment of wounds and ulcerae has been tested in a cell culture system. Phagocytosis of collagen fragments by human blood monocytes/macrophages has been investigated. For the assessment of collagen ingestion by mononuclear phagocytes, a picrosirius dye specific for collagen molecules has been used. By adapting this histochemical technique to microplate cell culture system, replicate monocyte cultures are assayed. Collagen content is determined by evaluating spectrophotometrically at 540 nm the absorbance of a sirius red/picric acid solution. Using this simple and sensitive method, the phagocytosis of bovine collagen by LPS-stimulated monocytes/macrophages has been ascertained.",
keywords = "Collagen, Monocytes, Phagocytosis, Picrosirius",
author = "G. Ciapetti and E. Verri and D. Granchi and E. Cenni and S. Gamberini and D. Benetti and M. Mian and A. Pizzoferrato",
year = "1996",
month = "9",
doi = "10.1016/0142-9612(96)87650-2",
language = "English",
volume = "17",
pages = "1703--1707",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "17",

}

TY - JOUR

T1 - In vitro assessment of phagocytosis of bovine collagen by human monocytes/macrophages using a spectrophotometric method

AU - Ciapetti, G.

AU - Verri, E.

AU - Granchi, D.

AU - Cenni, E.

AU - Gamberini, S.

AU - Benetti, D.

AU - Mian, M.

AU - Pizzoferrato, A.

PY - 1996/9

Y1 - 1996/9

N2 - The use of a wound dressing with covering and haemostatic properties significantly improves wound healing. In this study, a lyophilized bovine collagen sponge used for the treatment of wounds and ulcerae has been tested in a cell culture system. Phagocytosis of collagen fragments by human blood monocytes/macrophages has been investigated. For the assessment of collagen ingestion by mononuclear phagocytes, a picrosirius dye specific for collagen molecules has been used. By adapting this histochemical technique to microplate cell culture system, replicate monocyte cultures are assayed. Collagen content is determined by evaluating spectrophotometrically at 540 nm the absorbance of a sirius red/picric acid solution. Using this simple and sensitive method, the phagocytosis of bovine collagen by LPS-stimulated monocytes/macrophages has been ascertained.

AB - The use of a wound dressing with covering and haemostatic properties significantly improves wound healing. In this study, a lyophilized bovine collagen sponge used for the treatment of wounds and ulcerae has been tested in a cell culture system. Phagocytosis of collagen fragments by human blood monocytes/macrophages has been investigated. For the assessment of collagen ingestion by mononuclear phagocytes, a picrosirius dye specific for collagen molecules has been used. By adapting this histochemical technique to microplate cell culture system, replicate monocyte cultures are assayed. Collagen content is determined by evaluating spectrophotometrically at 540 nm the absorbance of a sirius red/picric acid solution. Using this simple and sensitive method, the phagocytosis of bovine collagen by LPS-stimulated monocytes/macrophages has been ascertained.

KW - Collagen

KW - Monocytes

KW - Phagocytosis

KW - Picrosirius

UR - http://www.scopus.com/inward/record.url?scp=0030248176&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030248176&partnerID=8YFLogxK

U2 - 10.1016/0142-9612(96)87650-2

DO - 10.1016/0142-9612(96)87650-2

M3 - Article

C2 - 8866032

AN - SCOPUS:0030248176

VL - 17

SP - 1703

EP - 1707

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 17

ER -