In vitro assessment of phagocytosis of bovine collagen by human monocytes/macrophages using a spectrophotometric method

G. Ciapetti, E. Verri, D. Granchi, E. Cenni, S. Gamberini, D. Benetti, M. Mian, A. Pizzoferrato

Research output: Contribution to journalArticle

Abstract

The use of a wound dressing with covering and haemostatic properties significantly improves wound healing. In this study, a lyophilized bovine collagen sponge used for the treatment of wounds and ulcerae has been tested in a cell culture system. Phagocytosis of collagen fragments by human blood monocytes/macrophages has been investigated. For the assessment of collagen ingestion by mononuclear phagocytes, a picrosirius dye specific for collagen molecules has been used. By adapting this histochemical technique to microplate cell culture system, replicate monocyte cultures are assayed. Collagen content is determined by evaluating spectrophotometrically at 540 nm the absorbance of a sirius red/picric acid solution. Using this simple and sensitive method, the phagocytosis of bovine collagen by LPS-stimulated monocytes/macrophages has been ascertained.

Original languageEnglish
Pages (from-to)1703-1707
Number of pages5
JournalBiomaterials
Volume17
Issue number17
DOIs
Publication statusPublished - Sep 1996

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Keywords

  • Collagen
  • Monocytes
  • Phagocytosis
  • Picrosirius

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

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