In vitro assessment of radiobiology of meningioma: A pilot study

V. Pinzi, I. Bisogno, E. Ciusani, A. Canazza, C. Calatozzolo, I. G. Vetrano, F. Pasi, E. De Martin, M. L. Fumagalli, R. Nano, L. Fariselli

Research output: Contribution to journalArticle

Abstract

Background: Meningioma are the second most common brain tumors in adults and can cause significant morbidity and mortality. The scarcity of in vitro and in vivo models represents the major obstacle to understand the molecular basis of meningioma tumorigenesis. The main aim of this study was to assess a method for radiobiology of meningioma cells colture by means of well-known meningioma lines. New method: We carried out a protocol of cells culture for irradiation of meningioma cells. We used the immortalized cell lines IOMM-Lee and CH-157 to study their radiation-reponse by means of clonogenic assays and to evaluate their proliferation and apoptosis. We irradiated the cells with different total doses using two different linear accelerators. Results: We observed a more radiation resistance of the IOMM-Lee than the CH-157. Indeed, the cellular death of CH-157 was obtained at a very low dose irradiation. Moreover, we showed a dose-response effect due to the early and late apoptosis, in fact the rate of apoptotic cells is greater than that of the necrotic cells at any dose of irradiation and at any time of analysis. Comparison with existing methods: There is not a standardized method for radiobiology of meningioma experiments. Conclusions: Our method of cells culture appears suitable for radiosensitivity studies on meningioma. We can confirm that the response to radiotherapy depends not only on irradiation features, but also on tumor radiosensitivity.

Original languageEnglish
Pages (from-to)288-294
Number of pages7
JournalJournal of Neuroscience Methods
Volume311
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Radiobiology
Meningioma
Radiation Tolerance
Cell Culture Techniques
Radiation
Apoptosis
Particle Accelerators
In Vitro Techniques
Brain Neoplasms
Carcinogenesis
Radiotherapy
Morbidity
Cell Line
Mortality

Keywords

  • Cell cultures
  • Meningioma
  • Meningioma cells
  • Radiobiology
  • Radiosensitivity
  • Survival curves

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

In vitro assessment of radiobiology of meningioma : A pilot study. / Pinzi, V.; Bisogno, I.; Ciusani, E.; Canazza, A.; Calatozzolo, C.; Vetrano, I. G.; Pasi, F.; De Martin, E.; Fumagalli, M. L.; Nano, R.; Fariselli, L.

In: Journal of Neuroscience Methods, Vol. 311, 01.01.2019, p. 288-294.

Research output: Contribution to journalArticle

@article{e47fe9b64e6e4195aff0ca5d61860feb,
title = "In vitro assessment of radiobiology of meningioma: A pilot study",
abstract = "Background: Meningioma are the second most common brain tumors in adults and can cause significant morbidity and mortality. The scarcity of in vitro and in vivo models represents the major obstacle to understand the molecular basis of meningioma tumorigenesis. The main aim of this study was to assess a method for radiobiology of meningioma cells colture by means of well-known meningioma lines. New method: We carried out a protocol of cells culture for irradiation of meningioma cells. We used the immortalized cell lines IOMM-Lee and CH-157 to study their radiation-reponse by means of clonogenic assays and to evaluate their proliferation and apoptosis. We irradiated the cells with different total doses using two different linear accelerators. Results: We observed a more radiation resistance of the IOMM-Lee than the CH-157. Indeed, the cellular death of CH-157 was obtained at a very low dose irradiation. Moreover, we showed a dose-response effect due to the early and late apoptosis, in fact the rate of apoptotic cells is greater than that of the necrotic cells at any dose of irradiation and at any time of analysis. Comparison with existing methods: There is not a standardized method for radiobiology of meningioma experiments. Conclusions: Our method of cells culture appears suitable for radiosensitivity studies on meningioma. We can confirm that the response to radiotherapy depends not only on irradiation features, but also on tumor radiosensitivity.",
keywords = "Cell cultures, Meningioma, Meningioma cells, Radiobiology, Radiosensitivity, Survival curves",
author = "V. Pinzi and I. Bisogno and E. Ciusani and A. Canazza and C. Calatozzolo and Vetrano, {I. G.} and F. Pasi and {De Martin}, E. and Fumagalli, {M. L.} and R. Nano and L. Fariselli",
year = "2019",
month = "1",
day = "1",
doi = "10.1016/j.jneumeth.2018.11.003",
language = "English",
volume = "311",
pages = "288--294",
journal = "Journal of Neuroscience Methods",
issn = "0165-0270",
publisher = "Elsevier",

}

TY - JOUR

T1 - In vitro assessment of radiobiology of meningioma

T2 - A pilot study

AU - Pinzi, V.

AU - Bisogno, I.

AU - Ciusani, E.

AU - Canazza, A.

AU - Calatozzolo, C.

AU - Vetrano, I. G.

AU - Pasi, F.

AU - De Martin, E.

AU - Fumagalli, M. L.

AU - Nano, R.

AU - Fariselli, L.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Background: Meningioma are the second most common brain tumors in adults and can cause significant morbidity and mortality. The scarcity of in vitro and in vivo models represents the major obstacle to understand the molecular basis of meningioma tumorigenesis. The main aim of this study was to assess a method for radiobiology of meningioma cells colture by means of well-known meningioma lines. New method: We carried out a protocol of cells culture for irradiation of meningioma cells. We used the immortalized cell lines IOMM-Lee and CH-157 to study their radiation-reponse by means of clonogenic assays and to evaluate their proliferation and apoptosis. We irradiated the cells with different total doses using two different linear accelerators. Results: We observed a more radiation resistance of the IOMM-Lee than the CH-157. Indeed, the cellular death of CH-157 was obtained at a very low dose irradiation. Moreover, we showed a dose-response effect due to the early and late apoptosis, in fact the rate of apoptotic cells is greater than that of the necrotic cells at any dose of irradiation and at any time of analysis. Comparison with existing methods: There is not a standardized method for radiobiology of meningioma experiments. Conclusions: Our method of cells culture appears suitable for radiosensitivity studies on meningioma. We can confirm that the response to radiotherapy depends not only on irradiation features, but also on tumor radiosensitivity.

AB - Background: Meningioma are the second most common brain tumors in adults and can cause significant morbidity and mortality. The scarcity of in vitro and in vivo models represents the major obstacle to understand the molecular basis of meningioma tumorigenesis. The main aim of this study was to assess a method for radiobiology of meningioma cells colture by means of well-known meningioma lines. New method: We carried out a protocol of cells culture for irradiation of meningioma cells. We used the immortalized cell lines IOMM-Lee and CH-157 to study their radiation-reponse by means of clonogenic assays and to evaluate their proliferation and apoptosis. We irradiated the cells with different total doses using two different linear accelerators. Results: We observed a more radiation resistance of the IOMM-Lee than the CH-157. Indeed, the cellular death of CH-157 was obtained at a very low dose irradiation. Moreover, we showed a dose-response effect due to the early and late apoptosis, in fact the rate of apoptotic cells is greater than that of the necrotic cells at any dose of irradiation and at any time of analysis. Comparison with existing methods: There is not a standardized method for radiobiology of meningioma experiments. Conclusions: Our method of cells culture appears suitable for radiosensitivity studies on meningioma. We can confirm that the response to radiotherapy depends not only on irradiation features, but also on tumor radiosensitivity.

KW - Cell cultures

KW - Meningioma

KW - Meningioma cells

KW - Radiobiology

KW - Radiosensitivity

KW - Survival curves

UR - http://www.scopus.com/inward/record.url?scp=85056212879&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85056212879&partnerID=8YFLogxK

U2 - 10.1016/j.jneumeth.2018.11.003

DO - 10.1016/j.jneumeth.2018.11.003

M3 - Article

C2 - 30408557

AN - SCOPUS:85056212879

VL - 311

SP - 288

EP - 294

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

SN - 0165-0270

ER -