In vitro assessment of radiobiology of meningioma: A pilot study

V. Pinzi, I. Bisogno, E. Ciusani, A. Canazza, C. Calatozzolo, I.G. Vetrano, F. Pasi, E. De Martin, M.L. Fumagalli, R. Nano, L. Fariselli

Research output: Contribution to journalArticle

Abstract

Background Meningioma are the second most common brain tumors in adults and can cause significant morbidity and mortality. The scarcity of in vitro and in vivo models represents the major obstacle to understand the molecular basis of meningioma tumorigenesis. The main aim of this study was to assess a method for radiobiology of meningioma cells colture by means of well-known meningioma lines. New method We carried out a protocol of cells culture for irradiation of meningioma cells. We used the immortalized cell lines IOMM-Lee and CH-157 to study their radiation-reponse by means of clonogenic assays and to evaluate their proliferation and apoptosis. We irradiated the cells with different total doses using two different linear accelerators. Results We observed a more radiation resistance of the IOMM-Lee than the CH-157. Indeed, the cellular death of CH-157 was obtained at a very low dose irradiation. Moreover, we showed a dose-response effect due to the early and late apoptosis, in fact the rate of apoptotic cells is greater than that of the necrotic cells at any dose of irradiation and at any time of analysis. Comparison with existing methods There is not a standardized method for radiobiology of meningioma experiments. Conclusions Our method of cells culture appears suitable for radiosensitivity studies on meningioma. We can confirm that the response to radiotherapy depends not only on irradiation features, but also on tumor radiosensitivity.
Original languageEnglish
Pages (from-to)288-294
Number of pages7
JournalJournal of Neuroscience Methods
Volume311
DOIs
Publication statusPublished - 2019

Keywords

  • Meningioma
  • Meningioma cells
  • Radiobiology
  • Radiosensitivity
  • Survival curves
  • Cell cultures

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