In vitro calcified matrix deposition by human osteoblasts onto a zinc-containing bioactive glass

Enrica Saino, Stefania Grandi, Eliana Quartarone, Valentina Maliardi, Daniela Galli, Nora Bloise, Lorenzo Fassina, Maria Gabriella Cusella de Angelis, Piercarlo Mustarelli, Marcello Imbriani, Livia Visai

Research output: Contribution to journalArticlepeer-review


Bioactive glasses synthesized by the sol-gel technique possess many of the qualities associated with an ideal scaffold material for a bone graft substitute. In view of the potential clinical applications, we performed a detailed in vitro study of the biological reactivity of synthesized 58S bioactive glass containing-zinc, in terms of osteoblast morphology, proliferation, and deposition of a mineralized extracellular matrix (ECM). Human Sarcoma Osteoblast (SAOS-2) cells were used to i) assess cytotoxicity by lactate dehydrogenase (LDH) release and ii) evaluate the deposition of a calcified extracellular matrix by ELISA assay and quantitative RT-PCR (qRT-PCR). In comparison with pure silica and 58S, the 58S-Zn0.4 bioglass showed a significant increase in cellular proliferation and deposition of ECM components such as decorin, fibronectin, osteocalcin, osteonectin, osteopontin, type-I and -IIIcollagens. Calcium deposition was significantly higher than on pure silica and 58S samples. Also Alkaline phosphatase(ALP) activity and its protein content was higher with respect to pure silica and 58S. qRT-PCR analysis revealed the up-regulation of type-I collagen, bone sialoprotein and osteopontin genes. All together these results demonstratethe cytocompatibility of 58S-Zn0.4 bioglass and its capability to promote osteoblast differentiation.

Original languageEnglish
Pages (from-to)59-72
Number of pages14
JournalEuropean Cells and Materials
Publication statusPublished - Jan 2011


  • Extracellular matrix deposition
  • Mineralization
  • Osteoblasts
  • Silica
  • Sol-gel bioactive glass
  • Zinc-containing bioactive glass

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Bioengineering
  • Biomedical Engineering
  • Biomaterials


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