TY - JOUR
T1 - In vitro calcified matrix deposition by human osteoblasts onto a zinc-containing bioactive glass
AU - Saino, Enrica
AU - Grandi, Stefania
AU - Quartarone, Eliana
AU - Maliardi, Valentina
AU - Galli, Daniela
AU - Bloise, Nora
AU - Fassina, Lorenzo
AU - de Angelis, Maria Gabriella Cusella
AU - Mustarelli, Piercarlo
AU - Imbriani, Marcello
AU - Visai, Livia
PY - 2011/1
Y1 - 2011/1
N2 - Bioactive glasses synthesized by the sol-gel technique possess many of the qualities associated with an ideal scaffold material for a bone graft substitute. In view of the potential clinical applications, we performed a detailed in vitro study of the biological reactivity of synthesized 58S bioactive glass containing-zinc, in terms of osteoblast morphology, proliferation, and deposition of a mineralized extracellular matrix (ECM). Human Sarcoma Osteoblast (SAOS-2) cells were used to i) assess cytotoxicity by lactate dehydrogenase (LDH) release and ii) evaluate the deposition of a calcified extracellular matrix by ELISA assay and quantitative RT-PCR (qRT-PCR). In comparison with pure silica and 58S, the 58S-Zn0.4 bioglass showed a significant increase in cellular proliferation and deposition of ECM components such as decorin, fibronectin, osteocalcin, osteonectin, osteopontin, type-I and -IIIcollagens. Calcium deposition was significantly higher than on pure silica and 58S samples. Also Alkaline phosphatase(ALP) activity and its protein content was higher with respect to pure silica and 58S. qRT-PCR analysis revealed the up-regulation of type-I collagen, bone sialoprotein and osteopontin genes. All together these results demonstratethe cytocompatibility of 58S-Zn0.4 bioglass and its capability to promote osteoblast differentiation.
AB - Bioactive glasses synthesized by the sol-gel technique possess many of the qualities associated with an ideal scaffold material for a bone graft substitute. In view of the potential clinical applications, we performed a detailed in vitro study of the biological reactivity of synthesized 58S bioactive glass containing-zinc, in terms of osteoblast morphology, proliferation, and deposition of a mineralized extracellular matrix (ECM). Human Sarcoma Osteoblast (SAOS-2) cells were used to i) assess cytotoxicity by lactate dehydrogenase (LDH) release and ii) evaluate the deposition of a calcified extracellular matrix by ELISA assay and quantitative RT-PCR (qRT-PCR). In comparison with pure silica and 58S, the 58S-Zn0.4 bioglass showed a significant increase in cellular proliferation and deposition of ECM components such as decorin, fibronectin, osteocalcin, osteonectin, osteopontin, type-I and -IIIcollagens. Calcium deposition was significantly higher than on pure silica and 58S samples. Also Alkaline phosphatase(ALP) activity and its protein content was higher with respect to pure silica and 58S. qRT-PCR analysis revealed the up-regulation of type-I collagen, bone sialoprotein and osteopontin genes. All together these results demonstratethe cytocompatibility of 58S-Zn0.4 bioglass and its capability to promote osteoblast differentiation.
KW - Extracellular matrix deposition
KW - Mineralization
KW - Osteoblasts
KW - Silica
KW - Sol-gel bioactive glass
KW - Zinc-containing bioactive glass
UR - http://www.scopus.com/inward/record.url?scp=79551568033&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79551568033&partnerID=8YFLogxK
M3 - Article
C2 - 21240845
AN - SCOPUS:79551568033
VL - 21
SP - 59
EP - 72
JO - European Cells and Materials
JF - European Cells and Materials
SN - 1473-2262
ER -