In vitro cell cultures obtained from different explants of Corylus avellana produce Taxol and taxanes

Federica Bestoso, Laura Ottaggio, Andrea Armirotti, Alessandro Balbi, Gianluca Damonte, Paolo Degan, Mauro Mazzei, Francesca Cavalli, Bernardetta Ledda, Mariangela Miele

Research output: Contribution to journalArticle

Abstract

Background: Taxol is an effective antineoplastic agent, originally extracted from the bark of Taxus brevifolia with a low yield. Many attempts have been made to produce Taxol by chemical synthesis, semi-synthesis and plant tissue cultures. However, to date, the availability of this compound is not sufficient to satisfy the commercial requirements. The aim of the present work was to produce suspension cell cultures from plants not belonging to Taxus genus and to verify whether they produced Taxol and taxanes. For this purpose different explants of hazel (Corylus avellana species) were used to optimize the protocol for inducing in vitro callus, an undifferentiated tissue from which suspension cell cultures were established. Results: Calli were successfully induced from stems, leaves and seeds grown in various hormone concentrations and combinations. The most suitable callus to establish suspension cell cultures was obtained from seeds. Media recovered from suspension cell cultures contained taxanes, and showed antiproliferative activity on human tumour cells. Taxol, 10-deacetyltaxol and 10-deacetylbaccatin III were the main taxanes identified. The level of Taxol recovered from the media of hazel cultures was similar to that found in yew cultures. Moreover, the production of taxanes in hazel cell cultures increased when elicitors were used. Conclusion: Here we show that hazel cell cultures produce Taxol and taxanes under controlled conditions. This result suggests that hazel possesses the enzymes for Taxol production, which until now was considered to be a pathway particular to Taxus genus. The main benefit of producing taxanes through hazel cell cultures is that hazel is widely available, grows at a much faster rate in vivo, and is easier to cultivate in vitro than yew. In addition, the production of callus directly from hazel seeds shortens the culture time and minimizes the probability of contamination. Therefore, hazel could become a commercial source of Taxol and taxanes, both to be used as new therapeutic agents or as new precursors for Taxol semi-synthesis.

Original languageEnglish
Article number45
JournalBMC Biotechnology
Volume6
DOIs
Publication statusPublished - Dec 6 2006

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Corylus
Taxoids
Paclitaxel
Cell Culture Techniques
Bony Callus
Taxus
Suspensions
Seeds
In Vitro Techniques
Cotyledon
Human Activities
Antineoplastic Agents
Culture Media
Hormones

ASJC Scopus subject areas

  • Medicine(all)
  • Biotechnology

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In vitro cell cultures obtained from different explants of Corylus avellana produce Taxol and taxanes. / Bestoso, Federica; Ottaggio, Laura; Armirotti, Andrea; Balbi, Alessandro; Damonte, Gianluca; Degan, Paolo; Mazzei, Mauro; Cavalli, Francesca; Ledda, Bernardetta; Miele, Mariangela.

In: BMC Biotechnology, Vol. 6, 45, 06.12.2006.

Research output: Contribution to journalArticle

Bestoso, F, Ottaggio, L, Armirotti, A, Balbi, A, Damonte, G, Degan, P, Mazzei, M, Cavalli, F, Ledda, B & Miele, M 2006, 'In vitro cell cultures obtained from different explants of Corylus avellana produce Taxol and taxanes', BMC Biotechnology, vol. 6, 45. https://doi.org/10.1186/1472-6750-6-45
Bestoso, Federica ; Ottaggio, Laura ; Armirotti, Andrea ; Balbi, Alessandro ; Damonte, Gianluca ; Degan, Paolo ; Mazzei, Mauro ; Cavalli, Francesca ; Ledda, Bernardetta ; Miele, Mariangela. / In vitro cell cultures obtained from different explants of Corylus avellana produce Taxol and taxanes. In: BMC Biotechnology. 2006 ; Vol. 6.
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abstract = "Background: Taxol is an effective antineoplastic agent, originally extracted from the bark of Taxus brevifolia with a low yield. Many attempts have been made to produce Taxol by chemical synthesis, semi-synthesis and plant tissue cultures. However, to date, the availability of this compound is not sufficient to satisfy the commercial requirements. The aim of the present work was to produce suspension cell cultures from plants not belonging to Taxus genus and to verify whether they produced Taxol and taxanes. For this purpose different explants of hazel (Corylus avellana species) were used to optimize the protocol for inducing in vitro callus, an undifferentiated tissue from which suspension cell cultures were established. Results: Calli were successfully induced from stems, leaves and seeds grown in various hormone concentrations and combinations. The most suitable callus to establish suspension cell cultures was obtained from seeds. Media recovered from suspension cell cultures contained taxanes, and showed antiproliferative activity on human tumour cells. Taxol, 10-deacetyltaxol and 10-deacetylbaccatin III were the main taxanes identified. The level of Taxol recovered from the media of hazel cultures was similar to that found in yew cultures. Moreover, the production of taxanes in hazel cell cultures increased when elicitors were used. Conclusion: Here we show that hazel cell cultures produce Taxol and taxanes under controlled conditions. This result suggests that hazel possesses the enzymes for Taxol production, which until now was considered to be a pathway particular to Taxus genus. The main benefit of producing taxanes through hazel cell cultures is that hazel is widely available, grows at a much faster rate in vivo, and is easier to cultivate in vitro than yew. In addition, the production of callus directly from hazel seeds shortens the culture time and minimizes the probability of contamination. Therefore, hazel could become a commercial source of Taxol and taxanes, both to be used as new therapeutic agents or as new precursors for Taxol semi-synthesis.",
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AU - Bestoso, Federica

AU - Ottaggio, Laura

AU - Armirotti, Andrea

AU - Balbi, Alessandro

AU - Damonte, Gianluca

AU - Degan, Paolo

AU - Mazzei, Mauro

AU - Cavalli, Francesca

AU - Ledda, Bernardetta

AU - Miele, Mariangela

PY - 2006/12/6

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N2 - Background: Taxol is an effective antineoplastic agent, originally extracted from the bark of Taxus brevifolia with a low yield. Many attempts have been made to produce Taxol by chemical synthesis, semi-synthesis and plant tissue cultures. However, to date, the availability of this compound is not sufficient to satisfy the commercial requirements. The aim of the present work was to produce suspension cell cultures from plants not belonging to Taxus genus and to verify whether they produced Taxol and taxanes. For this purpose different explants of hazel (Corylus avellana species) were used to optimize the protocol for inducing in vitro callus, an undifferentiated tissue from which suspension cell cultures were established. Results: Calli were successfully induced from stems, leaves and seeds grown in various hormone concentrations and combinations. The most suitable callus to establish suspension cell cultures was obtained from seeds. Media recovered from suspension cell cultures contained taxanes, and showed antiproliferative activity on human tumour cells. Taxol, 10-deacetyltaxol and 10-deacetylbaccatin III were the main taxanes identified. The level of Taxol recovered from the media of hazel cultures was similar to that found in yew cultures. Moreover, the production of taxanes in hazel cell cultures increased when elicitors were used. Conclusion: Here we show that hazel cell cultures produce Taxol and taxanes under controlled conditions. This result suggests that hazel possesses the enzymes for Taxol production, which until now was considered to be a pathway particular to Taxus genus. The main benefit of producing taxanes through hazel cell cultures is that hazel is widely available, grows at a much faster rate in vivo, and is easier to cultivate in vitro than yew. In addition, the production of callus directly from hazel seeds shortens the culture time and minimizes the probability of contamination. Therefore, hazel could become a commercial source of Taxol and taxanes, both to be used as new therapeutic agents or as new precursors for Taxol semi-synthesis.

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