Abstract
Spleen cell cultures from immunized and control mice have been followed in their morphological evolution, especially towards "blastoid" and binucleated cells. The immunological activity of cultures from immunized mice has been tested at different ages of culture by the Jerne antibody plaque essay. The ability of spleen cells to synthetize antibody was maintained in vitro for 15 days. DNA and RNA synthesis have been studied by radioautography using thymidine and uridine as precursors, respectively. No differences in the kinetic of synthesis appeared by relating the two types of cultures.
| Original language | Italian |
|---|---|
| Pages (from-to) | 364-377 |
| Number of pages | 14 |
| Journal | Zeitschrift für Zellforschung und Mikroskopische Anatomie |
| Volume | 98 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Sep 1969 |
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ASJC Scopus subject areas
- Anatomy
- Clinical Biochemistry
- Cell Biology
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Colture in vitro di cellule spleniche di topo - Risposta anticorpale e sintesi degli acidi nucleici. / Larizza, Libia.
In: Zeitschrift für Zellforschung und Mikroskopische Anatomie, Vol. 98, No. 3, 09.1969, p. 364-377.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Colture in vitro di cellule spleniche di topo - Risposta anticorpale e sintesi degli acidi nucleici
AU - Larizza, Libia
PY - 1969/9
Y1 - 1969/9
N2 - Spleen cell cultures from immunized and control mice have been followed in their morphological evolution, especially towards "blastoid" and binucleated cells. The immunological activity of cultures from immunized mice has been tested at different ages of culture by the Jerne antibody plaque essay. The ability of spleen cells to synthetize antibody was maintained in vitro for 15 days. DNA and RNA synthesis have been studied by radioautography using thymidine and uridine as precursors, respectively. No differences in the kinetic of synthesis appeared by relating the two types of cultures.
AB - Spleen cell cultures from immunized and control mice have been followed in their morphological evolution, especially towards "blastoid" and binucleated cells. The immunological activity of cultures from immunized mice has been tested at different ages of culture by the Jerne antibody plaque essay. The ability of spleen cells to synthetize antibody was maintained in vitro for 15 days. DNA and RNA synthesis have been studied by radioautography using thymidine and uridine as precursors, respectively. No differences in the kinetic of synthesis appeared by relating the two types of cultures.
UR - http://www.scopus.com/inward/record.url?scp=0014639981&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0014639981&partnerID=8YFLogxK
U2 - 10.1007/BF00346681
DO - 10.1007/BF00346681
M3 - Articolo
C2 - 5389075
AN - SCOPUS:0014639981
VL - 98
SP - 364
EP - 377
JO - Cell and Tissue Research
JF - Cell and Tissue Research
SN - 0302-766X
IS - 3
ER -