Colture in vitro di cellule spleniche di topo - Risposta anticorpale e sintesi degli acidi nucleici

Translated title of the contribution: In vitro cell cultures of the spleen of the mouse - Antibody response and synthesis of nucleic acids

Research output: Contribution to journalArticle

Abstract

Spleen cell cultures from immunized and control mice have been followed in their morphological evolution, especially towards "blastoid" and binucleated cells. The immunological activity of cultures from immunized mice has been tested at different ages of culture by the Jerne antibody plaque essay. The ability of spleen cells to synthetize antibody was maintained in vitro for 15 days. DNA and RNA synthesis have been studied by radioautography using thymidine and uridine as precursors, respectively. No differences in the kinetic of synthesis appeared by relating the two types of cultures.

Original languageItalian
Pages (from-to)364-377
Number of pages14
JournalZeitschrift für Zellforschung und Mikroskopische Anatomie
Volume98
Issue number3
DOIs
Publication statusPublished - Sep 1969

Fingerprint

Cell culture
Nucleic Acids
Antibody Formation
Spleen
Cell Culture Techniques
Antibodies
Uridine
Autoradiography
Thymidine
RNA
Kinetics
DNA
In Vitro Techniques

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

@article{d79464ea58fe44dba1b2077380d81df4,
title = "Colture in vitro di cellule spleniche di topo - Risposta anticorpale e sintesi degli acidi nucleici",
abstract = "Spleen cell cultures from immunized and control mice have been followed in their morphological evolution, especially towards {"}blastoid{"} and binucleated cells. The immunological activity of cultures from immunized mice has been tested at different ages of culture by the Jerne antibody plaque essay. The ability of spleen cells to synthetize antibody was maintained in vitro for 15 days. DNA and RNA synthesis have been studied by radioautography using thymidine and uridine as precursors, respectively. No differences in the kinetic of synthesis appeared by relating the two types of cultures.",
author = "Libia Larizza",
year = "1969",
month = "9",
doi = "10.1007/BF00346681",
language = "Italian",
volume = "98",
pages = "364--377",
journal = "Cell and Tissue Research",
issn = "0302-766X",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Colture in vitro di cellule spleniche di topo - Risposta anticorpale e sintesi degli acidi nucleici

AU - Larizza, Libia

PY - 1969/9

Y1 - 1969/9

N2 - Spleen cell cultures from immunized and control mice have been followed in their morphological evolution, especially towards "blastoid" and binucleated cells. The immunological activity of cultures from immunized mice has been tested at different ages of culture by the Jerne antibody plaque essay. The ability of spleen cells to synthetize antibody was maintained in vitro for 15 days. DNA and RNA synthesis have been studied by radioautography using thymidine and uridine as precursors, respectively. No differences in the kinetic of synthesis appeared by relating the two types of cultures.

AB - Spleen cell cultures from immunized and control mice have been followed in their morphological evolution, especially towards "blastoid" and binucleated cells. The immunological activity of cultures from immunized mice has been tested at different ages of culture by the Jerne antibody plaque essay. The ability of spleen cells to synthetize antibody was maintained in vitro for 15 days. DNA and RNA synthesis have been studied by radioautography using thymidine and uridine as precursors, respectively. No differences in the kinetic of synthesis appeared by relating the two types of cultures.

UR - http://www.scopus.com/inward/record.url?scp=0014639981&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0014639981&partnerID=8YFLogxK

U2 - 10.1007/BF00346681

DO - 10.1007/BF00346681

M3 - Articolo

C2 - 5389075

AN - SCOPUS:0014639981

VL - 98

SP - 364

EP - 377

JO - Cell and Tissue Research

JF - Cell and Tissue Research

SN - 0302-766X

IS - 3

ER -