In-vitro cell-mediated cytotoxicity for autologous liver cells in chronic non-A, non-B hepatitis

M. Mondelli, A. Alberti, F. Tremolada, R. Williams, A. L. Eddleston, G. Realdi

Research output: Contribution to journalArticle

Abstract

To investigate the possible mechanisms of liver cell injury in chronic non-A, non-B (NANB) hepatitis, peripheral blood lymphocytes (PBL) from 16 patients with chronic NANB hepatitis were incubated with autologous hepatocytes in a microcytotoxicity assay. Significant cytotoxicity was demonstrated in 11 patients. T-enriched lymphocytes exhibited significantly greater cytotoxicity than non-T enriched cells. No significant inhibition of cytotoxicity was observed following preincubation of the liver cells with either monoclonal or polyclonal anti-HBc, or monoclonal anti-HBs, or addition of either purified HBsAg or recombinant HBcAg to the culture, indicating that there was no detectable cross-reactivity in this system between hepatitis B virus (HBV) and NANB-associated antigen(s). Preincubation of the patients' hepatocytes with polyclonal IgG purified from a serum of a patient who recovered from an acute NANB hepatitis, did not significantly alter cytotoxicity. Liver cell surface-bound IgG was detected by immunofluoresence in only two of the patients, a finding consistent with existing evidence of poor antibody responses to both liver membrane and NANB-associated antigens. Control experiments using PBL from allogeneic normal donors exhibited normal cytotoxicity for the patients' hepatocytes supporting the hypothesis that antibody-dependent cell-mediated cytotoxicity (ADCC) is unlikely to play a significant role in this clinical setting.

Original languageEnglish
Pages (from-to)147-155
Number of pages9
JournalClinical and Experimental Immunology
Volume63
Issue number1
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Immunology

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    Mondelli, M., Alberti, A., Tremolada, F., Williams, R., Eddleston, A. L., & Realdi, G. (1986). In-vitro cell-mediated cytotoxicity for autologous liver cells in chronic non-A, non-B hepatitis. Clinical and Experimental Immunology, 63(1), 147-155.