Abstract
It was shown that Dacarbazine and other triazene compounds render murine leukemias highly immunogenic and susceptible to natural immunity (NI). In addition a pilot clinical study revealed that Dacarbazine can be cytotoxic for bone marrow blasts in patients with acute non-lymphoblastic leukemias through a mechanism that could be, at least in part, of immunological origin. However triazenes depress antigen-dependent responses and NI, whereas interferons, including interferon-beta (IFN), antagonize drug-induced impairment of NI. Therefore the complex interaction between triazenes and IFN on NI effector (i.e. NK) lymphocytes and human target lymphoblastoid cells has been investigated. the results show that: (a) EFN increases NK activity and antagonizes the depressive effects of methyl-triazene-benzoic acid (MTBA, an in vitro active triazene compound) on the NK function; (b) a lymphoblastoid cell line exposed to multiple in vitro treatments with MTBA, shows increased growth rate, augmented chemoresistance to MTBA, and higher susceptibility to NI than parental cells; (c) as expected IFN pretreatment down-regulates the susceptibility of lymphoblastoid cells to NK effectors; (d) however a net "therapeutic gain" was found if the overall influence of MTBA + IFN on target and effector cells is considered.
| Original language | English |
|---|---|
| Pages (from-to) | 695-715 |
| Number of pages | 21 |
| Journal | Immunopharmacology and Immunotoxicology |
| Volume | 16 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1994 |
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ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
- Pharmacology
- Toxicology
- Health, Toxicology and Mutagenesis
Cite this
In vitro combined effects of a triazene compound and interferon-beta on natural immunity against lymphoblastoid cells : Studies at effector and target cell level. / Bonmassar, L.; D'atri, S.; Turriziani, M.; Giuliani, A.
In: Immunopharmacology and Immunotoxicology, Vol. 16, No. 4, 1994, p. 695-715.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro combined effects of a triazene compound and interferon-beta on natural immunity against lymphoblastoid cells
T2 - Studies at effector and target cell level
AU - Bonmassar, L.
AU - D'atri, S.
AU - Turriziani, M.
AU - Giuliani, A.
PY - 1994
Y1 - 1994
N2 - It was shown that Dacarbazine and other triazene compounds render murine leukemias highly immunogenic and susceptible to natural immunity (NI). In addition a pilot clinical study revealed that Dacarbazine can be cytotoxic for bone marrow blasts in patients with acute non-lymphoblastic leukemias through a mechanism that could be, at least in part, of immunological origin. However triazenes depress antigen-dependent responses and NI, whereas interferons, including interferon-beta (IFN), antagonize drug-induced impairment of NI. Therefore the complex interaction between triazenes and IFN on NI effector (i.e. NK) lymphocytes and human target lymphoblastoid cells has been investigated. the results show that: (a) EFN increases NK activity and antagonizes the depressive effects of methyl-triazene-benzoic acid (MTBA, an in vitro active triazene compound) on the NK function; (b) a lymphoblastoid cell line exposed to multiple in vitro treatments with MTBA, shows increased growth rate, augmented chemoresistance to MTBA, and higher susceptibility to NI than parental cells; (c) as expected IFN pretreatment down-regulates the susceptibility of lymphoblastoid cells to NK effectors; (d) however a net "therapeutic gain" was found if the overall influence of MTBA + IFN on target and effector cells is considered.
AB - It was shown that Dacarbazine and other triazene compounds render murine leukemias highly immunogenic and susceptible to natural immunity (NI). In addition a pilot clinical study revealed that Dacarbazine can be cytotoxic for bone marrow blasts in patients with acute non-lymphoblastic leukemias through a mechanism that could be, at least in part, of immunological origin. However triazenes depress antigen-dependent responses and NI, whereas interferons, including interferon-beta (IFN), antagonize drug-induced impairment of NI. Therefore the complex interaction between triazenes and IFN on NI effector (i.e. NK) lymphocytes and human target lymphoblastoid cells has been investigated. the results show that: (a) EFN increases NK activity and antagonizes the depressive effects of methyl-triazene-benzoic acid (MTBA, an in vitro active triazene compound) on the NK function; (b) a lymphoblastoid cell line exposed to multiple in vitro treatments with MTBA, shows increased growth rate, augmented chemoresistance to MTBA, and higher susceptibility to NI than parental cells; (c) as expected IFN pretreatment down-regulates the susceptibility of lymphoblastoid cells to NK effectors; (d) however a net "therapeutic gain" was found if the overall influence of MTBA + IFN on target and effector cells is considered.
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UR - http://www.scopus.com/inward/citedby.url?scp=0028073450&partnerID=8YFLogxK
U2 - 10.3109/08923979409019746
DO - 10.3109/08923979409019746
M3 - Article
C2 - 7876468
AN - SCOPUS:0028073450
VL - 16
SP - 695
EP - 715
JO - Immunopharmacology and Immunotoxicology
JF - Immunopharmacology and Immunotoxicology
SN - 0892-3973
IS - 4
ER -