In vitro cytolytic activity of gastric juice from patients with different fasting intragastric bile acid concentrations

Maria Notarnicola, Francesco Russo, Pasquale Berloco, Dionigi Lorusso, Francesco Pezzolla, Alfredo Di Leo

Research output: Contribution to journalArticle

Abstract

Objective: Evaluation of the cytolytic effect of intragastric bile acids collected from postoperative patients with different degrees of duodenogastric reflux. Patients and Methods: The study included 60 patients, 26 of whom had undergone cholecystectomy for gallstones (group A), while the other 34 had undergone a Billroth II gastric resection for peptic ulcer disease (group B). Intragastric bile acid concentration was evaluated after a 12-h fast by drainage of gastric contents every 15 min for 1 h using a nasogastric tube. The total bile acid (TBA) concentrations were measured by an enzymatic method. Cytotoxicity of gastric juice was assessed by haemolysis of human erythrocytes and expressed as ü of gastric juice required for 50% haemolysis (C50). Results: Group B patients had higher intragastric TBA levels than those from group A (P<0.05). This was paralleled by the significantly greater in vitro cytolytic activity exhibited by gastric juice from group B compared with group A patients (P

Original languageEnglish
Pages (from-to)603-606
Number of pages4
JournalEuropean Journal of Gastroenterology and Hepatology
Volume6
Issue number7
Publication statusPublished - 1994

Keywords

  • Bile acids
  • Cytolytic activity
  • Duodenogastric reflux

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

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