In vitro cytotoxicity evaluation of cadmium by label-free holographic microscopy

Martina Mugnano, Pasquale Memmolo, Lisa Miccio, Simonetta Grilli, Francesco Merola, Alejandro Calabuig, Alessia Bramanti, Emanuela Mazzon, Pietro Ferraro

Research output: Contribution to journalArticle

Abstract

Among all environmental pollutants, the toxic heavy metal cadmium is considered as a human carcinogen. Cadmium may induce cell death by apoptosis in various cell types, although the underlying mechanisms are still unclear. In this paper we show how a label-free digital holography (DH)-based technique is able to quantify the evolution of key biophysical parameters of cells during the exposure to cadmium for the first time. Murine embryonic fibroblasts NIH 3T3 are chosen here as cellular model for studying the cadmium effects. The results demonstrate that DH is able to retrieve the temporal evolution of different key parameters such as cell volume, projected area, cell thickness and dry mass, thus providing a full quantitative characterization of the cell physical behaviour during cadmium exposure. Our results show that the label-free character of the technique would allow biologists to perform systematic and reliable studies on cell death process induced by cadmium and we believe that more in general this can be easily extended to others heavy metals, thus avoiding the time-consuming, expensive and invasive label-based procedures used nowadays in the field. In fact, pollution by heavy metals is severe issue that needs rapid and reliable methods to be settled.

Original languageEnglish
Pages (from-to)e201800099
JournalJournal of Biophotonics
Volume11
Issue number12
DOIs
Publication statusPublished - Dec 11 2018

Fingerprint

Cytotoxicity
Cadmium
cadmium
Labels
Microscopy
Microscopic examination
microscopy
evaluation
Cell death
Holography
heavy metals
Heavy Metals
Heavy metals
cells
death
holography
Cell Death
carcinogens
Environmental Pollutants
Carcinogens

Cite this

Mugnano, M., Memmolo, P., Miccio, L., Grilli, S., Merola, F., Calabuig, A., ... Ferraro, P. (2018). In vitro cytotoxicity evaluation of cadmium by label-free holographic microscopy. Journal of Biophotonics, 11(12), e201800099. https://doi.org/10.1002/jbio.201800099

In vitro cytotoxicity evaluation of cadmium by label-free holographic microscopy. / Mugnano, Martina; Memmolo, Pasquale; Miccio, Lisa; Grilli, Simonetta; Merola, Francesco; Calabuig, Alejandro; Bramanti, Alessia; Mazzon, Emanuela; Ferraro, Pietro.

In: Journal of Biophotonics, Vol. 11, No. 12, 11.12.2018, p. e201800099.

Research output: Contribution to journalArticle

Mugnano, M, Memmolo, P, Miccio, L, Grilli, S, Merola, F, Calabuig, A, Bramanti, A, Mazzon, E & Ferraro, P 2018, 'In vitro cytotoxicity evaluation of cadmium by label-free holographic microscopy', Journal of Biophotonics, vol. 11, no. 12, pp. e201800099. https://doi.org/10.1002/jbio.201800099
Mugnano M, Memmolo P, Miccio L, Grilli S, Merola F, Calabuig A et al. In vitro cytotoxicity evaluation of cadmium by label-free holographic microscopy. Journal of Biophotonics. 2018 Dec 11;11(12):e201800099. https://doi.org/10.1002/jbio.201800099
Mugnano, Martina ; Memmolo, Pasquale ; Miccio, Lisa ; Grilli, Simonetta ; Merola, Francesco ; Calabuig, Alejandro ; Bramanti, Alessia ; Mazzon, Emanuela ; Ferraro, Pietro. / In vitro cytotoxicity evaluation of cadmium by label-free holographic microscopy. In: Journal of Biophotonics. 2018 ; Vol. 11, No. 12. pp. e201800099.
@article{f09ba0e616b248a3bd1d99f974d3a833,
title = "In vitro cytotoxicity evaluation of cadmium by label-free holographic microscopy",
abstract = "Among all environmental pollutants, the toxic heavy metal cadmium is considered as a human carcinogen. Cadmium may induce cell death by apoptosis in various cell types, although the underlying mechanisms are still unclear. In this paper we show how a label-free digital holography (DH)-based technique is able to quantify the evolution of key biophysical parameters of cells during the exposure to cadmium for the first time. Murine embryonic fibroblasts NIH 3T3 are chosen here as cellular model for studying the cadmium effects. The results demonstrate that DH is able to retrieve the temporal evolution of different key parameters such as cell volume, projected area, cell thickness and dry mass, thus providing a full quantitative characterization of the cell physical behaviour during cadmium exposure. Our results show that the label-free character of the technique would allow biologists to perform systematic and reliable studies on cell death process induced by cadmium and we believe that more in general this can be easily extended to others heavy metals, thus avoiding the time-consuming, expensive and invasive label-based procedures used nowadays in the field. In fact, pollution by heavy metals is severe issue that needs rapid and reliable methods to be settled.",
author = "Martina Mugnano and Pasquale Memmolo and Lisa Miccio and Simonetta Grilli and Francesco Merola and Alejandro Calabuig and Alessia Bramanti and Emanuela Mazzon and Pietro Ferraro",
note = "{\circledC} 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",
year = "2018",
month = "12",
day = "11",
doi = "10.1002/jbio.201800099",
language = "English",
volume = "11",
pages = "e201800099",
journal = "Journal of Biophotonics",
issn = "1864-063X",
publisher = "Wiley-VCH Verlag",
number = "12",

}

TY - JOUR

T1 - In vitro cytotoxicity evaluation of cadmium by label-free holographic microscopy

AU - Mugnano, Martina

AU - Memmolo, Pasquale

AU - Miccio, Lisa

AU - Grilli, Simonetta

AU - Merola, Francesco

AU - Calabuig, Alejandro

AU - Bramanti, Alessia

AU - Mazzon, Emanuela

AU - Ferraro, Pietro

N1 - © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2018/12/11

Y1 - 2018/12/11

N2 - Among all environmental pollutants, the toxic heavy metal cadmium is considered as a human carcinogen. Cadmium may induce cell death by apoptosis in various cell types, although the underlying mechanisms are still unclear. In this paper we show how a label-free digital holography (DH)-based technique is able to quantify the evolution of key biophysical parameters of cells during the exposure to cadmium for the first time. Murine embryonic fibroblasts NIH 3T3 are chosen here as cellular model for studying the cadmium effects. The results demonstrate that DH is able to retrieve the temporal evolution of different key parameters such as cell volume, projected area, cell thickness and dry mass, thus providing a full quantitative characterization of the cell physical behaviour during cadmium exposure. Our results show that the label-free character of the technique would allow biologists to perform systematic and reliable studies on cell death process induced by cadmium and we believe that more in general this can be easily extended to others heavy metals, thus avoiding the time-consuming, expensive and invasive label-based procedures used nowadays in the field. In fact, pollution by heavy metals is severe issue that needs rapid and reliable methods to be settled.

AB - Among all environmental pollutants, the toxic heavy metal cadmium is considered as a human carcinogen. Cadmium may induce cell death by apoptosis in various cell types, although the underlying mechanisms are still unclear. In this paper we show how a label-free digital holography (DH)-based technique is able to quantify the evolution of key biophysical parameters of cells during the exposure to cadmium for the first time. Murine embryonic fibroblasts NIH 3T3 are chosen here as cellular model for studying the cadmium effects. The results demonstrate that DH is able to retrieve the temporal evolution of different key parameters such as cell volume, projected area, cell thickness and dry mass, thus providing a full quantitative characterization of the cell physical behaviour during cadmium exposure. Our results show that the label-free character of the technique would allow biologists to perform systematic and reliable studies on cell death process induced by cadmium and we believe that more in general this can be easily extended to others heavy metals, thus avoiding the time-consuming, expensive and invasive label-based procedures used nowadays in the field. In fact, pollution by heavy metals is severe issue that needs rapid and reliable methods to be settled.

U2 - 10.1002/jbio.201800099

DO - 10.1002/jbio.201800099

M3 - Article

VL - 11

SP - e201800099

JO - Journal of Biophotonics

JF - Journal of Biophotonics

SN - 1864-063X

IS - 12

ER -