In vitro cytotoxicity of orthodontic primers.

Vincenzo D'Antò, Gianrico Spagnuolo, Ilaria Polito, Sergio Paduano, Luigi Ambrosio, Rosa Valletta

Research output: Contribution to journalArticlepeer-review


OBJECTIVES: The aim of this study was to evaluate the cytotoxicity of four orthodontic primers: Transbond XT and Transbond MIP (3M, USA), Eagle Fluorsure (American Orthodontics, USA) and Ortho Solo (Ormco, USA). MATERIALS AND METHODS: Balb 3T3 cells were exposed to different concentrations of primers (0-0.25 mg/ml). Mitochondrial dehydrogenase activity was evaluated by MTT assay and cell necrosis was measured by flow cytometry (propidium iodide staining). RESULTS: All the materials decreased cell viability in a dose related manner. Cytotoxicity of orthodontic primers based on concentrations which caused a 50% decrease of mitochondrial activity was ranked as follows: Transbond XT (45.57 mg/ml) > Eagle Fluorsure (49.27 mg/ml) > Transbond MIP (64.35 mg/ml) > Ortho solo (70.09 mg/ml). CONCLUSIONS: Our results suggest that the cytotoxic potencies demonstrated by orthodontic primers might be of clinical relevance since they disturbed cell metabolism and induced cell death in monolayer cultures.

Original languageEnglish
Pages (from-to)4-11
Number of pages8
JournalProgress in Orthodontics
Issue number1
Publication statusPublished - 2009

ASJC Scopus subject areas

  • Medicine(all)


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