Abstract
Objective: To compare directly in vitro developmental competence between parthenogenetically activated and intracytoplasmic sperm injection (ICSI)-fertilized oocytes. Design: For each patient, three metaphase II oocytes were randomized to the ICSI procedure, while n-3 were allocated to parthenogenetic activation. Setting: University hospital infertility unit. Patients: Thirty-eight patients, aged 35.2 ± 3.3 years (mean ± SD) selected for ICSI. Interventions: After 1 hour from denudation, oocytes were either fertilized by ICSI (n = 114) or chemically activated (n = 104). Fertilized and activated oocytes were cultured for up to 3 and 5 days, respectively. Main Outcome Measures: Development rate, cell number, and morphological grade during culture. Results: The two groups showed no significant differences between rates of fertilization and parthenogenetic activation, development, and blastomere number on days 2 and 3 of culture. However, parthenotes showed a lower morphological grade, and a significantly lower proportion went on cleaving to day 3, when only activated rather than total numbers of oocytes were considered. On day 5 after activation, nine oocytes (8.6%) reached the blastocyst stage, representing 12.9% of parthenotes. Conclusions: Since most parameters examined in this study were similar between activated and fertilized oocytes, parthenogenetic activation may be a useful tool for the preclinical evaluation of experimental procedures.
| Original language | English |
|---|---|
| Pages (from-to) | 77-82 |
| Number of pages | 6 |
| Journal | Fertility and Sterility |
| Volume | 87 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2007 |
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Keywords
- blastocyst
- ICSI
- in vitro development
- Parthenogenesis
ASJC Scopus subject areas
- Obstetrics and Gynaecology
Cite this
In vitro development of human oocytes after parthenogenetic activation or intracytoplasmic sperm injection. / Paffoni, Alessio; Brevini, Tiziana A L; Somigliana, Edgardo; Restelli, Liliana; Gandolfi, Fulvio; Ragni, Guido.
In: Fertility and Sterility, Vol. 87, No. 1, 01.2007, p. 77-82.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro development of human oocytes after parthenogenetic activation or intracytoplasmic sperm injection
AU - Paffoni, Alessio
AU - Brevini, Tiziana A L
AU - Somigliana, Edgardo
AU - Restelli, Liliana
AU - Gandolfi, Fulvio
AU - Ragni, Guido
PY - 2007/1
Y1 - 2007/1
N2 - Objective: To compare directly in vitro developmental competence between parthenogenetically activated and intracytoplasmic sperm injection (ICSI)-fertilized oocytes. Design: For each patient, three metaphase II oocytes were randomized to the ICSI procedure, while n-3 were allocated to parthenogenetic activation. Setting: University hospital infertility unit. Patients: Thirty-eight patients, aged 35.2 ± 3.3 years (mean ± SD) selected for ICSI. Interventions: After 1 hour from denudation, oocytes were either fertilized by ICSI (n = 114) or chemically activated (n = 104). Fertilized and activated oocytes were cultured for up to 3 and 5 days, respectively. Main Outcome Measures: Development rate, cell number, and morphological grade during culture. Results: The two groups showed no significant differences between rates of fertilization and parthenogenetic activation, development, and blastomere number on days 2 and 3 of culture. However, parthenotes showed a lower morphological grade, and a significantly lower proportion went on cleaving to day 3, when only activated rather than total numbers of oocytes were considered. On day 5 after activation, nine oocytes (8.6%) reached the blastocyst stage, representing 12.9% of parthenotes. Conclusions: Since most parameters examined in this study were similar between activated and fertilized oocytes, parthenogenetic activation may be a useful tool for the preclinical evaluation of experimental procedures.
AB - Objective: To compare directly in vitro developmental competence between parthenogenetically activated and intracytoplasmic sperm injection (ICSI)-fertilized oocytes. Design: For each patient, three metaphase II oocytes were randomized to the ICSI procedure, while n-3 were allocated to parthenogenetic activation. Setting: University hospital infertility unit. Patients: Thirty-eight patients, aged 35.2 ± 3.3 years (mean ± SD) selected for ICSI. Interventions: After 1 hour from denudation, oocytes were either fertilized by ICSI (n = 114) or chemically activated (n = 104). Fertilized and activated oocytes were cultured for up to 3 and 5 days, respectively. Main Outcome Measures: Development rate, cell number, and morphological grade during culture. Results: The two groups showed no significant differences between rates of fertilization and parthenogenetic activation, development, and blastomere number on days 2 and 3 of culture. However, parthenotes showed a lower morphological grade, and a significantly lower proportion went on cleaving to day 3, when only activated rather than total numbers of oocytes were considered. On day 5 after activation, nine oocytes (8.6%) reached the blastocyst stage, representing 12.9% of parthenotes. Conclusions: Since most parameters examined in this study were similar between activated and fertilized oocytes, parthenogenetic activation may be a useful tool for the preclinical evaluation of experimental procedures.
KW - blastocyst
KW - ICSI
KW - in vitro development
KW - Parthenogenesis
UR - http://www.scopus.com/inward/record.url?scp=33845864179&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845864179&partnerID=8YFLogxK
U2 - 10.1016/j.fertnstert.2006.05.063
DO - 10.1016/j.fertnstert.2006.05.063
M3 - Article
C2 - 17074324
AN - SCOPUS:33845864179
VL - 87
SP - 77
EP - 82
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 1
ER -