TY - JOUR
T1 - In vitro development of human oocytes after parthenogenetic activation or intracytoplasmic sperm injection
AU - Paffoni, Alessio
AU - Brevini, Tiziana A L
AU - Somigliana, Edgardo
AU - Restelli, Liliana
AU - Gandolfi, Fulvio
AU - Ragni, Guido
PY - 2007/1
Y1 - 2007/1
N2 - Objective: To compare directly in vitro developmental competence between parthenogenetically activated and intracytoplasmic sperm injection (ICSI)-fertilized oocytes. Design: For each patient, three metaphase II oocytes were randomized to the ICSI procedure, while n-3 were allocated to parthenogenetic activation. Setting: University hospital infertility unit. Patients: Thirty-eight patients, aged 35.2 ± 3.3 years (mean ± SD) selected for ICSI. Interventions: After 1 hour from denudation, oocytes were either fertilized by ICSI (n = 114) or chemically activated (n = 104). Fertilized and activated oocytes were cultured for up to 3 and 5 days, respectively. Main Outcome Measures: Development rate, cell number, and morphological grade during culture. Results: The two groups showed no significant differences between rates of fertilization and parthenogenetic activation, development, and blastomere number on days 2 and 3 of culture. However, parthenotes showed a lower morphological grade, and a significantly lower proportion went on cleaving to day 3, when only activated rather than total numbers of oocytes were considered. On day 5 after activation, nine oocytes (8.6%) reached the blastocyst stage, representing 12.9% of parthenotes. Conclusions: Since most parameters examined in this study were similar between activated and fertilized oocytes, parthenogenetic activation may be a useful tool for the preclinical evaluation of experimental procedures.
AB - Objective: To compare directly in vitro developmental competence between parthenogenetically activated and intracytoplasmic sperm injection (ICSI)-fertilized oocytes. Design: For each patient, three metaphase II oocytes were randomized to the ICSI procedure, while n-3 were allocated to parthenogenetic activation. Setting: University hospital infertility unit. Patients: Thirty-eight patients, aged 35.2 ± 3.3 years (mean ± SD) selected for ICSI. Interventions: After 1 hour from denudation, oocytes were either fertilized by ICSI (n = 114) or chemically activated (n = 104). Fertilized and activated oocytes were cultured for up to 3 and 5 days, respectively. Main Outcome Measures: Development rate, cell number, and morphological grade during culture. Results: The two groups showed no significant differences between rates of fertilization and parthenogenetic activation, development, and blastomere number on days 2 and 3 of culture. However, parthenotes showed a lower morphological grade, and a significantly lower proportion went on cleaving to day 3, when only activated rather than total numbers of oocytes were considered. On day 5 after activation, nine oocytes (8.6%) reached the blastocyst stage, representing 12.9% of parthenotes. Conclusions: Since most parameters examined in this study were similar between activated and fertilized oocytes, parthenogenetic activation may be a useful tool for the preclinical evaluation of experimental procedures.
KW - blastocyst
KW - ICSI
KW - in vitro development
KW - Parthenogenesis
UR - http://www.scopus.com/inward/record.url?scp=33845864179&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845864179&partnerID=8YFLogxK
U2 - 10.1016/j.fertnstert.2006.05.063
DO - 10.1016/j.fertnstert.2006.05.063
M3 - Article
C2 - 17074324
AN - SCOPUS:33845864179
VL - 87
SP - 77
EP - 82
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 1
ER -