In vitro diagnosis of coeliac disease: An assessment

P. D. Howdle, G. R. Corazza, A. W. Bullen, M. S. Losowsky

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Jejunal biopsies from controls and coeliac patients were maintained in organ culture for up to 48 hours. The in vitro effect of gluten fraction III during the period of culture was assessed by measurement of the activity of the brush border enzymes alkaline phosphatase and α-glucosidase. Mucosa from controls and treated and untreated coeliacs behaved similarly and no reproducible in vitro effect of gluten was demonstrated. These results cast doubt on the in vitro diagnosis of coeliac disease by monitoring brush border enzyme activity.

Original languageEnglish
Pages (from-to)939-947
Number of pages9
JournalGut
Volume22
Issue number11
Publication statusPublished - 1981

Fingerprint

Celiac Disease
Glutens
Microvilli
Abdomen
Glucosidases
Organ Culture Techniques
Enzymes
Alkaline Phosphatase
Mucous Membrane
Biopsy
In Vitro Techniques

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Howdle, P. D., Corazza, G. R., Bullen, A. W., & Losowsky, M. S. (1981). In vitro diagnosis of coeliac disease: An assessment. Gut, 22(11), 939-947.

In vitro diagnosis of coeliac disease : An assessment. / Howdle, P. D.; Corazza, G. R.; Bullen, A. W.; Losowsky, M. S.

In: Gut, Vol. 22, No. 11, 1981, p. 939-947.

Research output: Contribution to journalArticle

Howdle, PD, Corazza, GR, Bullen, AW & Losowsky, MS 1981, 'In vitro diagnosis of coeliac disease: An assessment', Gut, vol. 22, no. 11, pp. 939-947.
Howdle PD, Corazza GR, Bullen AW, Losowsky MS. In vitro diagnosis of coeliac disease: An assessment. Gut. 1981;22(11):939-947.
Howdle, P. D. ; Corazza, G. R. ; Bullen, A. W. ; Losowsky, M. S. / In vitro diagnosis of coeliac disease : An assessment. In: Gut. 1981 ; Vol. 22, No. 11. pp. 939-947.
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