In vitro DNA and nuclear proteins alkylation by 1,2-dimethylhydrazine

Lidia C. Boffa, Claudia Bolognesi

Research output: Contribution to journalArticle

Abstract

The methylating carcinogen 1,2-dimethylhydrazine (DMH) CAS 540.73.8 is highly organ-specific and, under certain experimental conditions, produces a high incidence of adenocarcinoma in the colon of rodents. We have tried to assess the possibility that part of the organ-specifity in the carcinogenic effect of DMH could be attributed to its metabolism by specific microsomal enzymes. In particular, we compared the in vitro effects of DMH in the presence of either colon or liver microsomes from animals that had been treated with microsomal enzyme inducers. V79 Chinese hamster cells were used as the target to evaluate the damage to the genetic material, as judged by (1) formation of adducts of DNA bases and (2) amino acid modifications in nuclear proteins using [Me-14C]DMH and appropriate analytical detection systems. Our results tend to support the above postulated hypothesis.

Original languageEnglish
Pages (from-to)157-162
Number of pages6
JournalMutation Research Letters
Volume173
Issue number3
DOIs
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Genetics
  • Toxicology
  • Medicine(all)

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