In vitro effect of bombesin-related peptides on the procoagulant activity of alveolar macrophages

F. Meloni, A. Saporiti, P. Ballabio, A. Brocchieri, G. Grignani, G. Gialdroni Grassi

Research output: Contribution to journalArticlepeer-review

Abstract

Bombesin-related peptides (BRP) are present in the lung during foetal life and are mitogenic for normal bronchial epithelial cells, pulmonary fibroblasts, and small-cell lung carcinoma cell lines. Increased levels of BRP have been described in the adult lung of cigarette smokers and in smoking related lung diseases. BRP have also been involved in the network of neuroimmune interactions, having been shown to modulate the phagocytic function of monocytes and alveolar macrophages. BRP have recently been shown to modulate the release of procoagulant activity (PCA) by human monocytes and alveolar macrophages after a 24 h culture. The aim of this study was to evaluate the effect of bombesin on cell-associated PCA of alveolar macrophages (AMs) obtained from asymptomatic subjects. In basal conditions, AMs were found to possess a low procoagulant activity, that was not affected by their preincubation (4 h at 37°C) with phorbol myristate acetate (1 μg.mL-1). On the contrary, endotoxin lipopolysaccharide (LPS)) induced a significant increase of procoagulant activity of macrophages when used at a concentration of 1 μg.mL-1, in the same experimental conditions; whilst a lower (1 ng.mL-1) concentration of LPS nonsignificantly enhanced cell-associated PCA. Treatment of AMs with synthetic bombesin (BN) alone (10-6 to 10-10 M) did not enhance cell-associated PCA, whilst a significant effect was seen when BN was added to the lower concentration of LPS (BN 10-6 M + LPS 1 ng.mL-1: 12.6 U.10-6 cells; LPS alone 1 ng.mL-1: 7.8 U.10-6 cells). These results confirm that BRP can interfere with the activation of AMs, by potentiating the effect of LPS, and suggest a role for these peptides in smoke-induced activation of AM.

Original languageEnglish
Pages (from-to)187-190
Number of pages4
JournalMonaldi Archives for Chest Disease - Cardiac Series
Volume50
Issue number3
Publication statusPublished - 1995

Keywords

  • Alveolar macrophages
  • Bombesin related peptides
  • Procoagulant activity

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Pulmonary and Respiratory Medicine

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