“In vitro” Effect of Different Follicle—Stimulating Hormone Preparations on Sertoli Cells: Toward a Personalized Treatment for Male Infertility

Iva Arato, Giuseppe Grande, Ferran Barrachina, Catia Bellucci, Cinzia Lilli, Meritxell Jodar, Maria Chiara Aglietti, Francesca Mancini, Federica Vincenzoni, Alfredo Pontecorvi, Riccardo Calafiore, Rafael Oliva, Giovanni Luca, Francesca Mancuso, Domenico Milardi

Research output: Contribution to journalArticlepeer-review

Abstract

Follicle-stimulating hormone (FSH), a major regulator of spermatogenesis, has a crucial function in the development and function of the testis and it is extensively given as a fertility treatment to stimulate spermatogenesis. We analyzed the effects of different FSH preparations (α-follitropin, β-follitropin, and urofollitropin) in combination with testosterone on porcine pre-pubertal Sertoli cells. To study the effect of the different FSH treatments in the Sertoli cell function we performed Real Time PCR analysis of AMH, inhibin B, and FSH-r, an ELISA assay for AMH and inhibin B, and a high-throughput comparative proteomic analysis. We verified that all three preparations induced a reduction of AMH in terms of mRNA and secreted proteins, and an increase of inhibin B in terms of mRNA in all the FSH formulations, while solely α-follitropin produced an increase of secreted inhibin B in the culture medium. Comparative proteomic analysis of the three FSH preparations identified 46 proteins, 11 up-regulated and 2 down-regulated. Surprisingly, the combination of testosterone with β-follitropin specifically induced an up-regulation of eight specific secreted proteins. Our study, showing that the three different FSH preparations induce different effects, could offer the opportunity to shed light inside new applications to a personalized reproductive medicine.

Original languageEnglish
Article number401
JournalFrontiers in Endocrinology
Volume11
DOIs
Publication statusPublished - Jun 18 2020

Keywords

  • alpha follitropin
  • beta follitropin
  • proteomic analysis
  • Sertoli cells
  • urofollitropin

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism

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