In-vitro effect of retinoic acid on normal and chronic myeloid leukemia granulopoiesis

Massimo Aglietta, Wanda Piacibello, Alessandra Stacchini, Fiorella Sanavio, Felice Gavosto

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of increasing concentrations of retinoic acid (RA) on the in-vitro proliferation of normal and chronic myeloid leukemia (CML) granulo-monocyte precursors (CFU-GM) was studied. 10-7 M RA added to semisolid cultures stimulated the growth of day 14 but not of day 7 normal CFU-GM, whereas in CML the growth of both populations was either unchanged or inhibited. Five-day and 10-day preincubation of normal bone marrow cells with RA augmented the number of day 14 CFU-GM (by up to 187% with 10-6 M RA), whereas there was a marked decrease when CML cells were used. Total cellularity was not much affected, though a slight increase in liquid normal bone marrow cultures and a slight fall in CML cultures could be detected. These data point to a difference in the response to RA of normal and CML precursors. They may offer of preclinical basis for its employment to delay the blastic progression of CML.

Original languageEnglish
Pages (from-to)879-883
Number of pages5
JournalLeukemia Research
Volume9
Issue number7
DOIs
Publication statusPublished - 1985

Keywords

  • CFU-GM
  • Chronic myeloid leukemia
  • granulopoiesis
  • in vitro
  • retinoic acid

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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