In vitro effect of suloctidil on 14C-5-hydroxytryptamine uptake and liberation in rat platelets

G. de Gaetano, M. C. Roncaglioni, G. Miragliotta, M. Wielosz, S. Garattini

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Abstract

Suloctidil, a new drug endowed with antispasmodic and platelet function suppressing activities, has been shown to interfere in in vitro systems with the transport and the storage of 14C-5-HT in rat platelets. Suloctidil inhibited the uptake of 14C-5-HT and induced 14C-5-HT loss from platelets. The inhibition of 14C-5-HT uptake appeared to be either non-competitive or un-competitive, according to the concentration of the drug used. The effect of suloctidil in inducing 14C-5-HT loss from platelets increased by increasing the concentration of the drug, the time and the temperature of incubation with platelets. It was inhibited neither by an inhibitory of 5-HT uptake (chlorimipramine) nor by an inhibitor of the platelet release reaction (acetylsalicylic acid) nor by an excess of cold 5-HT in the incubation medium. Lactate dehydrogenase loss was slight from platelets incubated with suloctidil. The observed effects of suloctidil differed in several respects from those exhibited by imipramine, (+)-fenfluramine, (+)-amphetamine and 4-chloroamphetamine.

Original languageEnglish
Pages (from-to)315-324
Number of pages10
JournalPharmacological Research Communications
Volume9
Issue number4
DOIs
Publication statusPublished - 1977

Fingerprint

Suloctidil
Serotonin
Blood Platelets
Pharmaceutical Preparations
Fenfluramine
Clomipramine
Parasympatholytics
Imipramine
Platelet Aggregation Inhibitors
Amphetamine
In Vitro Techniques
L-Lactate Dehydrogenase
Aspirin
Temperature

ASJC Scopus subject areas

  • Pharmacology

Cite this

In vitro effect of suloctidil on 14C-5-hydroxytryptamine uptake and liberation in rat platelets. / de Gaetano, G.; Roncaglioni, M. C.; Miragliotta, G.; Wielosz, M.; Garattini, S.

In: Pharmacological Research Communications, Vol. 9, No. 4, 1977, p. 315-324.

Research output: Contribution to journalArticle

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