In Vitro Effectiveness of Microspheres Based on Silk Sericin and Chlorella vulgaris or Arthrospira platensis for Wound Healing Applications.

ELia Bari, Carla Renata Arciola, Barbara Vigani, Barbara Crivelli, Paola Moro, Giorgio Marrubini, Milena Sorrenti, Laura Catenacci, Giovanna Bruni, Theodora Chlapanidas, Enrico Lucarelli, Sara Perteghella, Maria Luisa Torre

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Some natural compounds have recently been widely employed in wound healing applications due to their biological properties. One such compound is sericin, which is produced by Bombix mori, while active polyphenols, polysaccharides and proteins are synthetized by Chlorella vulgaris and Arthrospira platensis microalgae. Our hypothesis was that sericin, as an optimal bioactive polymeric carrier for microencapsulation process, could also improve the regenerative effect of the microalgae. A solvent-free extraction method and spray drying technique were combined to obtain five formulations, based on algal extracts (C. vulgaris and A. platensis, Chl and Art, respectively) or silk sericin (Ser) or their mixtures (Chl-Ser and Art-Ser). The spray drying was a suitable method to produce microspheres with similar dimensions, characterized by collapsed morphology with a rough surface. Art and Art-Ser showed higher antioxidant properties than other formulations. All microspheres resulted in cytocompatibility on fibroblasts until 1.25 mg/mL and promoted cell migration and the complete wound closure; this positive effect was further highlighted after treatment with Art and Art-Ser. To our surprize the combination of sericin to Art did not improve the microalgae extract efficacy, at least in our experimental conditions.
Original languageEnglish
Pages (from-to)1-17
Number of pages17
JournalMaterials
Volume10
Issue number9
DOIs
Publication statusPublished - Aug 23 2017

Fingerprint

Sericins
Spirulina
Spray drying
Silk
Microspheres
Microencapsulation
Fibroblasts
Polysaccharides
Antioxidants
Proteins
Polyphenols

Keywords

  • Arthrospira platensis
  • Chlorella vulgaris
  • silk sericin
  • spray drying
  • wound healing

Cite this

In Vitro Effectiveness of Microspheres Based on Silk Sericin and Chlorella vulgaris or Arthrospira platensis for Wound Healing Applications. / Bari, ELia; Arciola, Carla Renata; Vigani, Barbara; Crivelli, Barbara; Moro, Paola; Marrubini, Giorgio; Sorrenti, Milena; Catenacci, Laura; Bruni, Giovanna; Chlapanidas, Theodora; Lucarelli, Enrico; Perteghella, Sara; Torre, Maria Luisa.

In: Materials, Vol. 10, No. 9, 23.08.2017, p. 1-17.

Research output: Contribution to journalArticle

Bari, EL, Arciola, CR, Vigani, B, Crivelli, B, Moro, P, Marrubini, G, Sorrenti, M, Catenacci, L, Bruni, G, Chlapanidas, T, Lucarelli, E, Perteghella, S & Torre, ML 2017, 'In Vitro Effectiveness of Microspheres Based on Silk Sericin and Chlorella vulgaris or Arthrospira platensis for Wound Healing Applications.', Materials, vol. 10, no. 9, pp. 1-17. https://doi.org/10.3390/ma10090983
Bari, ELia ; Arciola, Carla Renata ; Vigani, Barbara ; Crivelli, Barbara ; Moro, Paola ; Marrubini, Giorgio ; Sorrenti, Milena ; Catenacci, Laura ; Bruni, Giovanna ; Chlapanidas, Theodora ; Lucarelli, Enrico ; Perteghella, Sara ; Torre, Maria Luisa. / In Vitro Effectiveness of Microspheres Based on Silk Sericin and Chlorella vulgaris or Arthrospira platensis for Wound Healing Applications. In: Materials. 2017 ; Vol. 10, No. 9. pp. 1-17.
@article{b7f8932fb4294415b7ec3cf2a631dee5,
title = "In Vitro Effectiveness of Microspheres Based on Silk Sericin and Chlorella vulgaris or Arthrospira platensis for Wound Healing Applications.",
abstract = "Some natural compounds have recently been widely employed in wound healing applications due to their biological properties. One such compound is sericin, which is produced by Bombix mori, while active polyphenols, polysaccharides and proteins are synthetized by Chlorella vulgaris and Arthrospira platensis microalgae. Our hypothesis was that sericin, as an optimal bioactive polymeric carrier for microencapsulation process, could also improve the regenerative effect of the microalgae. A solvent-free extraction method and spray drying technique were combined to obtain five formulations, based on algal extracts (C. vulgaris and A. platensis, Chl and Art, respectively) or silk sericin (Ser) or their mixtures (Chl-Ser and Art-Ser). The spray drying was a suitable method to produce microspheres with similar dimensions, characterized by collapsed morphology with a rough surface. Art and Art-Ser showed higher antioxidant properties than other formulations. All microspheres resulted in cytocompatibility on fibroblasts until 1.25 mg/mL and promoted cell migration and the complete wound closure; this positive effect was further highlighted after treatment with Art and Art-Ser. To our surprize the combination of sericin to Art did not improve the microalgae extract efficacy, at least in our experimental conditions.",
keywords = "Arthrospira platensis, Chlorella vulgaris, silk sericin, spray drying, wound healing",
author = "ELia Bari and Arciola, {Carla Renata} and Barbara Vigani and Barbara Crivelli and Paola Moro and Giorgio Marrubini and Milena Sorrenti and Laura Catenacci and Giovanna Bruni and Theodora Chlapanidas and Enrico Lucarelli and Sara Perteghella and Torre, {Maria Luisa}",
year = "2017",
month = "8",
day = "23",
doi = "10.3390/ma10090983",
language = "English",
volume = "10",
pages = "1--17",
journal = "Materials",
issn = "1996-1944",
publisher = "MDPI AG",
number = "9",

}

TY - JOUR

T1 - In Vitro Effectiveness of Microspheres Based on Silk Sericin and Chlorella vulgaris or Arthrospira platensis for Wound Healing Applications.

AU - Bari, ELia

AU - Arciola, Carla Renata

AU - Vigani, Barbara

AU - Crivelli, Barbara

AU - Moro, Paola

AU - Marrubini, Giorgio

AU - Sorrenti, Milena

AU - Catenacci, Laura

AU - Bruni, Giovanna

AU - Chlapanidas, Theodora

AU - Lucarelli, Enrico

AU - Perteghella, Sara

AU - Torre, Maria Luisa

PY - 2017/8/23

Y1 - 2017/8/23

N2 - Some natural compounds have recently been widely employed in wound healing applications due to their biological properties. One such compound is sericin, which is produced by Bombix mori, while active polyphenols, polysaccharides and proteins are synthetized by Chlorella vulgaris and Arthrospira platensis microalgae. Our hypothesis was that sericin, as an optimal bioactive polymeric carrier for microencapsulation process, could also improve the regenerative effect of the microalgae. A solvent-free extraction method and spray drying technique were combined to obtain five formulations, based on algal extracts (C. vulgaris and A. platensis, Chl and Art, respectively) or silk sericin (Ser) or their mixtures (Chl-Ser and Art-Ser). The spray drying was a suitable method to produce microspheres with similar dimensions, characterized by collapsed morphology with a rough surface. Art and Art-Ser showed higher antioxidant properties than other formulations. All microspheres resulted in cytocompatibility on fibroblasts until 1.25 mg/mL and promoted cell migration and the complete wound closure; this positive effect was further highlighted after treatment with Art and Art-Ser. To our surprize the combination of sericin to Art did not improve the microalgae extract efficacy, at least in our experimental conditions.

AB - Some natural compounds have recently been widely employed in wound healing applications due to their biological properties. One such compound is sericin, which is produced by Bombix mori, while active polyphenols, polysaccharides and proteins are synthetized by Chlorella vulgaris and Arthrospira platensis microalgae. Our hypothesis was that sericin, as an optimal bioactive polymeric carrier for microencapsulation process, could also improve the regenerative effect of the microalgae. A solvent-free extraction method and spray drying technique were combined to obtain five formulations, based on algal extracts (C. vulgaris and A. platensis, Chl and Art, respectively) or silk sericin (Ser) or their mixtures (Chl-Ser and Art-Ser). The spray drying was a suitable method to produce microspheres with similar dimensions, characterized by collapsed morphology with a rough surface. Art and Art-Ser showed higher antioxidant properties than other formulations. All microspheres resulted in cytocompatibility on fibroblasts until 1.25 mg/mL and promoted cell migration and the complete wound closure; this positive effect was further highlighted after treatment with Art and Art-Ser. To our surprize the combination of sericin to Art did not improve the microalgae extract efficacy, at least in our experimental conditions.

KW - Arthrospira platensis

KW - Chlorella vulgaris

KW - silk sericin

KW - spray drying

KW - wound healing

U2 - 10.3390/ma10090983

DO - 10.3390/ma10090983

M3 - Article

VL - 10

SP - 1

EP - 17

JO - Materials

JF - Materials

SN - 1996-1944

IS - 9

ER -