In vitro effects of PEMFs on bone cell cultures of normal and osteopenic rat

Research output: Contribution to journalArticle

Abstract

The aim of this study was to examine the effects of low-frequency, low-energy pulsed electromagnetic fields (PEMFs) on cell proliferation and differentiation in rat osteoblast primary cultures. Cells were obtained from normal and osteopenic rat bone and were named NB and OB, respectively. The osteoblastic phenotype was assessed by stimulation with 1,25(OH2) vitamin D3. NB and OB cells were seeded in multiwell plates and exposed to PEMFs for two different periods. Control cultures of both groups were incubated under the same conditions, with the pulse generator off. Assessment of PEMF effects was performed for the following parameters for each culture: alkaline phosphatase (ALP) activity, osteocalcin level, and MTT test. Results showed that OB and NB cell proliferation was significantly improved (p <0.03, p = 0.04 respectively) after 48 h of PEMF exposure. Osteocalcin production of OB after 5 days of PEMF exposure was significantly higher than normal (p = 0.007) and osteopenic (p = 0.033) bone-derived controls. These results show that PEMFs act on osteopenic bone-derived osteoblasts, stimulating proliferation of cells and then, after a longer exposure, activating them.

Original languageEnglish
Pages (from-to)359-365
Number of pages7
JournalElectromagnetic Biology and Medicine
Volume19
Issue number3
Publication statusPublished - 2000

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Electromagnetic Fields
cell culture
Cell Culture Techniques
bones
Bone and Bones
rats
cell proliferation
osteocalcin
osteoblasts
Osteocalcin
Cell Proliferation
Osteoblasts
cholecalciferol
Cholecalciferol
cell differentiation
Alkaline Phosphatase
alkaline phosphatase
In Vitro Techniques
electromagnetic field
Cell Differentiation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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In vitro effects of PEMFs on bone cell cultures of normal and osteopenic rat. / Torricelli, P.; Fini, M.; Giavaresi, G.; Giardino, R.

In: Electromagnetic Biology and Medicine, Vol. 19, No. 3, 2000, p. 359-365.

Research output: Contribution to journalArticle

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