In vitro effects of saccharin on cell-mediated host defence mechanisms

Alberto Mantovani, Walter Luini, Gian Paolo Candiani, Mario Salmona, Federico Spreafico, Silvio Garattini

Research output: Contribution to journalArticlepeer-review


In vitro exposure to saccharin (0.1-2 mg/ml) did not affect the tumoricidal activity of macrophages and natural killer (NK) cells from mice and rats. In contrast saccharin at doses of 0.5-2 mg/ml significantly and consistently depressed the blastogenic response of rat lymph node cells to phytohemagglutinin (PHA). Similarly mouse splenocytes showed impaired responsiveness to PHA and bacterial lipopolysaccharides in the presence of 2 mg/ml saccharin. Compared with murine lymphoid cells, human peripheral blood lymphocytes were relatively resistant to the immunodepressive activity of saccharin, a low but significant, depression (21%) of the response to PHA being observed only at the highest concentration (2 mg/ml) in 1 of 3 normal healthy donors tested.

Original languageEnglish
Pages (from-to)287-295
Number of pages9
JournalToxicology Letters
Issue number3-4
Publication statusPublished - 1980

ASJC Scopus subject areas

  • Toxicology


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