In vitro effects of tire debris organic extracts on human spermatozoa

Michela Geminiani, Elena Moretti, Francesco Cetta, Cesare Castellini, Gaia Terzuoli, Lucia Mazzi, Giulia Collodel

Research output: Contribution to journalArticlepeer-review


Background & objectives: Environmental pollutants have detrimental effects on the reproductive function of human being and of animals. This study was aimed at evaluating the effects of tire debris, a fraction of particulate matter derived from tire wear, on motility and morphology of human spermatozoa. Methods: spermatozoa from ten healthy men were incubated for 4 hours with 10, 50 and 75 μg/ml of tire debris organic extract (TDOE). Sperm motility and morphology were evaluated according to WHO guidelines; the presence of apoptosis and necrosis was assessed by the AnnexinV/Propidium iodide assay. Ultrastructure of sperm organelles was observed by transmission electron microscopy. Results: incubation with 10 μg/ml of TDOE did not affect sperm quality, except for the value of apoptosis (median: 8% vs. 6%; P

Original languageEnglish
Pages (from-to)8-13
Number of pages6
JournalOpen Andrology Journal
Publication statusPublished - 2011


  • Human semen quality
  • Particulate matter
  • TEM
  • Tire debris organic extracts

ASJC Scopus subject areas

  • Reproductive Medicine


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