In vitro evaluation of antimicrobial efficacy of endodontic irrigants

Alberto Dagna, Carla Renata Arciola, Filippo Florindi, Andrea Scribante, Enrica Saino, Livia Visai, Claudio Poggio

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: The objective of this study was to compare in vitro, by MTT assay, the antimicrobial efficacy of Niclor 5 (5% NaOCl solution), Cloreximid (0.2% chlorhexidine and 0.2% cetrimide solution), 3% hydrogen peroxide and 17% EDTA against two microorganisms associated with primary endodontic infections. Methods: Enterococcus faecalis and Streptococcus mutans strains were selected for this test. Freshly extracted single-rooted human teeth were endodontically treated, inoculated with bacterial strains and then divided into different groups, each of them rinsed with Niclor 5 (5% NaOCl solution), Cloreximid (0.2% chlorhexidine and 0.2% cetrimide solution), 3% hydrogen peroxide,17% EDTA and with 5% NaOCl solution (positive control). Results: Even though all the tested irrigating solutions demonstrated antibacterial effects against E. faecalis and S. mutans, the results were significantly different between the various groups. The greatest antimicrobial effects were observed in groups treated with 5% NaOCl and 17% EDTA. Interestingly, the effectiveness of EDTA could be ascribed to its capability of detaching biofilm from canal walls.

Original languageEnglish
Pages (from-to)914-919
Number of pages6
JournalInternational Journal of Artificial Organs
Volume34
Issue number9
DOIs
Publication statusPublished - Sep 2011

Keywords

  • Endodontic irrigating solutions
  • Enterococcus faecalis
  • MTT assay
  • Streptococcus mutans

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Bioengineering
  • Medicine (miscellaneous)

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