In vitro evaluation of cell/biomaterial interaction by MTT assay

G. Ciapetti, E. Cenni, L. Pratelli, A. Pizzoferrato

Research output: Contribution to journalArticle

Abstract

The tetrazolium-based colorimetric assay (MTT test) measures only in vitro living cells and the results are directly related to the number of viable cultured cells. It has been adopted in immunological investigations, cancer research and, recently, biocompatibility evaluation. We used the MTT method with minor modifications to fit it to an in vitro study of biomaterial-cell interactions. The MTT assay was confirmed to be feasible, rapid and reproducible. Moreover, it showed a good correlation with other in vitro proliferation assays, such as the 3H-thymidine uptake assay. By using the MTT method and the ASTM procedure for extracting biomaterials, we quantified the in vitro cell compatibility of different metals and polymers.

Original languageEnglish
Pages (from-to)359-364
Number of pages6
JournalBiomaterials
Volume14
Issue number5
DOIs
Publication statusPublished - 1993

Keywords

  • Cell-biomaterial interactions
  • cytotoxicity
  • MTT assay

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'In vitro evaluation of cell/biomaterial interaction by MTT assay'. Together they form a unique fingerprint.

  • Cite this