In vitro evidence of the synergistic interaction of ceftopibrole and other antibiotics against multidrug-resistant Gram-negative isolates

Ausilia Aprile, Carla Caio, Floriana Gona, Stefania Stefani, Maria Lina Mezzatesta

Research output: Contribution to journalArticle

Abstract

The purpose of the present study was to investigate the in vitro activity of ceftobiprole in combination with other antimicrobials against 27 selected Gram-negative isolates, including ESBL-producing E. coli and KPC-OXA-48-producing K. pneumoniae. Ceftobiprole activity in combination with amikacin, colistin, levofloxacin, piperacillin/tazobactam and rifampin was evaluated by time-kill curves and gradient-cross method (except colistin). Among the 27 strains tested with gradient strips most were resistant to ceftobiprole. Synergy was observed in some cases with piperacillin/tazobactam. There was at least one synergistic combination towards 9 isolates belonging to different species. No antagonism was observed with any of the antibiotic tested. In time-kill curves, performed for 12 selected isolates, synergistic interaction was more frequent, occurring with 32/60 combinations. The most interesting results of our study are the bactericidal effects of ceftobiprole in combination with colistin or piperacillin/tazobactam tested against Gram-negative isolates, including KPC and OXA-48-producing K. pneumoniae.

Original languageEnglish
Article number114884
JournalDiagnostic Microbiology and Infectious Disease
Volume95
Issue number4
DOIs
Publication statusPublished - Dec 2019

Keywords

  • Carbapenem-resistance
  • Ceftobiprole
  • Combination
  • Gram-negative
  • Time-kill curves

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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