In vitro exposure to human immunodeficiency virus type 1 induces apoptotic cell death of the factor-dependent TF-1 hematopoietic cell line

Giorgio Zauli, Marco Vitale, Maria Carla Re, Giuliano Furlini, Loris Zamai, Elisabetta Falcieri, Davide Gibellini, Giuseppe Visani, Brian R. Davis, Silvano Capitani, Michele La Placa

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Abstract

In this study, we evaluated the effect of a short-term exposure (2 hours) to two different lymphocytotropic strains of human immunodeficiency virus type 1 (HIV-1; HIV(IIIB) and ICR-3) on the survival of a factor-dependent CD34+ hematopoietic progenitor cell line (TF-1). At flow cytometry analysis, a significant (P <.05) increase in the frequency of apoptotic cell death was observed in HIV-1-treated TF-1 cells, supplemented with low doses of either interleukin-3 (IL-3; 0.02 to 1 ng/mL) or granulocyte-macrophage colony- stimulating factor (GM-CSF; 0.02 to 0.2 ng/mL) with respect to mock-treated cells. On the other hand, higher doses of both cytokines or combinations of suboptimal concentrations of IL-3 plus GM-CSF (eg, 0.2 plus 0.2 ng/mL) completely reversed the HIV-1-induced increase of apoptosis. Remarkably, no signs of productive or latent virus replication were ever observed in HIV-1- treated TF-1 cells up to 16 days of liquid culture. In parallel experiments, the in vitro exposure to HIV(IIIB) induced a significant and progressive increase of apoptotic death in purified bone marrow CD34+ cells, seeded in liquid cultures in the presence of 1 ng/mL IL-3. The HIV-1-induced apoptosis of TF-1 cells was likely triggered by the simple interaction of HIV-1 envelope glycoprotein gp120 with CD4 receptor, which was expressed at a low level on the surface of TF-1 cells. In fact, treatment of TF-1 cells with recombinant gp120 plus a polyclonal anti-gp120 antibody or with anti-CD4 monoclonal antibody plus rabbit anti-mouse IgG significantly increased the percentage of apoptotic death. These data suggest that HIV-1, and perhaps also free gp120 in the presence of anti-gp120 antibody, could play a direct role in the pathogenesis of peripheral blood cytopenias in acquired immunodeficiency syndrome patients by inducing apoptotic death of hematopoietic progenitor cells without the need of a direct infection.

Original languageEnglish
Pages (from-to)167-175
Number of pages9
JournalBlood
Volume83
Issue number1
Publication statusPublished - Jan 1 1994

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Interleukin-3
Cell death
Viruses
HIV-1
Cell Death
Granulocyte-Macrophage Colony-Stimulating Factor
Cells
Cell Line
Apoptosis
CD4 Antigens
Flow cytometry
Antibodies
Liquids
Hematopoietic Stem Cells
Anti-Idiotypic Antibodies
Glycoproteins
Bone
Blood
Immunoglobulin G
Monoclonal Antibodies

ASJC Scopus subject areas

  • Hematology

Cite this

Zauli, G., Vitale, M., Re, M. C., Furlini, G., Zamai, L., Falcieri, E., ... La Placa, M. (1994). In vitro exposure to human immunodeficiency virus type 1 induces apoptotic cell death of the factor-dependent TF-1 hematopoietic cell line. Blood, 83(1), 167-175.

In vitro exposure to human immunodeficiency virus type 1 induces apoptotic cell death of the factor-dependent TF-1 hematopoietic cell line. / Zauli, Giorgio; Vitale, Marco; Re, Maria Carla; Furlini, Giuliano; Zamai, Loris; Falcieri, Elisabetta; Gibellini, Davide; Visani, Giuseppe; Davis, Brian R.; Capitani, Silvano; La Placa, Michele.

In: Blood, Vol. 83, No. 1, 01.01.1994, p. 167-175.

Research output: Contribution to journalArticle

Zauli, G, Vitale, M, Re, MC, Furlini, G, Zamai, L, Falcieri, E, Gibellini, D, Visani, G, Davis, BR, Capitani, S & La Placa, M 1994, 'In vitro exposure to human immunodeficiency virus type 1 induces apoptotic cell death of the factor-dependent TF-1 hematopoietic cell line', Blood, vol. 83, no. 1, pp. 167-175.
Zauli, Giorgio ; Vitale, Marco ; Re, Maria Carla ; Furlini, Giuliano ; Zamai, Loris ; Falcieri, Elisabetta ; Gibellini, Davide ; Visani, Giuseppe ; Davis, Brian R. ; Capitani, Silvano ; La Placa, Michele. / In vitro exposure to human immunodeficiency virus type 1 induces apoptotic cell death of the factor-dependent TF-1 hematopoietic cell line. In: Blood. 1994 ; Vol. 83, No. 1. pp. 167-175.
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abstract = "In this study, we evaluated the effect of a short-term exposure (2 hours) to two different lymphocytotropic strains of human immunodeficiency virus type 1 (HIV-1; HIV(IIIB) and ICR-3) on the survival of a factor-dependent CD34+ hematopoietic progenitor cell line (TF-1). At flow cytometry analysis, a significant (P <.05) increase in the frequency of apoptotic cell death was observed in HIV-1-treated TF-1 cells, supplemented with low doses of either interleukin-3 (IL-3; 0.02 to 1 ng/mL) or granulocyte-macrophage colony- stimulating factor (GM-CSF; 0.02 to 0.2 ng/mL) with respect to mock-treated cells. On the other hand, higher doses of both cytokines or combinations of suboptimal concentrations of IL-3 plus GM-CSF (eg, 0.2 plus 0.2 ng/mL) completely reversed the HIV-1-induced increase of apoptosis. Remarkably, no signs of productive or latent virus replication were ever observed in HIV-1- treated TF-1 cells up to 16 days of liquid culture. In parallel experiments, the in vitro exposure to HIV(IIIB) induced a significant and progressive increase of apoptotic death in purified bone marrow CD34+ cells, seeded in liquid cultures in the presence of 1 ng/mL IL-3. The HIV-1-induced apoptosis of TF-1 cells was likely triggered by the simple interaction of HIV-1 envelope glycoprotein gp120 with CD4 receptor, which was expressed at a low level on the surface of TF-1 cells. In fact, treatment of TF-1 cells with recombinant gp120 plus a polyclonal anti-gp120 antibody or with anti-CD4 monoclonal antibody plus rabbit anti-mouse IgG significantly increased the percentage of apoptotic death. These data suggest that HIV-1, and perhaps also free gp120 in the presence of anti-gp120 antibody, could play a direct role in the pathogenesis of peripheral blood cytopenias in acquired immunodeficiency syndrome patients by inducing apoptotic death of hematopoietic progenitor cells without the need of a direct infection.",
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AU - Falcieri, Elisabetta

AU - Gibellini, Davide

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