In vitro generation of mechanically functional cartilage grafts based on adult human stem cells and 3D-woven poly(ε-caprolactone) scaffolds

Piia K. Valonen, Franklin T. Moutos, Akihiko Kusanagi, Matteo G. Moretti, Brian O. Diekman, Jean F. Welter, Arnold I. Caplan, Farshid Guilak, Lisa E. Freed

Research output: Contribution to journalArticle

Abstract

Three-dimensionally woven poly(ε-caprolactone) (PCL) scaffolds were combined with adult human mesenchymal stem cells (hMSC) to engineer mechanically functional cartilage constructs in vitro. The specific objectives were to: (i) produce PCL scaffolds with cartilage-like mechanical properties, (ii) demonstrate that hMSCs formed cartilage after 21 days of culture on PCL scaffolds, and (iii) study effects of scaffold structure (loosely vs. tightly woven), culture vessel (static dish vs. oscillating bioreactor), and medium composition (chondrogenic additives with or without serum). Aggregate moduli of 21-day constructs approached normal articular cartilage for tightly woven PCL cultured in bioreactors, were lower for tightly woven PCL cultured statically, and lowest for loosely woven PCL cultured statically (p 

Original languageEnglish
Pages (from-to)2193-2200
Number of pages8
JournalBiomaterials
Volume31
Issue number8
DOIs
Publication statusPublished - Mar 2010

Keywords

  • Biomechanics
  • Bioreactor
  • Cartilage repair
  • Growth factors
  • Stem cell

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

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