Génération des plaquettes in vitro : où en sommes-nous ?

Translated title of the contribution: In vitro generation of platelets: Where do we stand?

C. A. Di Buduo, D. L. Kaplan, A. Balduini

Research output: Contribution to journalShort surveypeer-review

Abstract

Millions of platelets, specialized cells that participate in haemostatic and inflammatory functions, are transfused each year worldwide, but their supply is limited. Platelets are produced by megakaryocytes by extending proplatelets, directly into the bloodstream. Bone marrow structure and extracellular matrix composition together with soluble factors (e.g. Thrombopoietin) are key regulators of megakaryopoiesis by supporting cell differentiation and platelet release. Despite this knowledge, the scarcity of clinical cures for life threatening platelet diseases is in a large part due to limited insight into the mechanisms that control the developmental process of megakaryocytes and the mechanisms that govern the production of platelets within the bone marrow. To overcome these limitations, functional human tissue models have been developed and studied to extrapolate ex vivo outcomes for new insight on bone marrow functions in vivo. There are many challenges that these models must overcome, from faithfully mimicking the physiological composition and functions of bone marrow, to the collection of the platelets generated and validation of their viability and function for human use. The overall goal is to identify innovative instruments to study mechanisms of platelet release, diseases related to platelet production and new therapeutic targets starting from human progenitor cells.

Translated title of the contributionIn vitro generation of platelets: Where do we stand?
Original languageFrench
Pages (from-to)273-276
Number of pages4
JournalTransfusion Clinique et Biologique
Volume24
Issue number3
DOIs
Publication statusPublished - Sep 1 2017

Keywords

  • 3D models
  • Bioreactor
  • Megakaryocytes
  • Platelets
  • Thrombocytopenia
  • Transfusion

ASJC Scopus subject areas

  • Hematology
  • Clinical Biochemistry
  • Biochemistry, medical

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