In vitro IL-6 production by EBV-immortalized B lymphocytes from young and elderly people genotyped for -174 C/G polymorphism in IL-6 gene: A model to study the genetic basis of inflamm-aging

Fabiola Olivieri, Massimiliano Bonafè, Simona Giovagnetti, Rosalia Stecconi, Maurizio Cardelli, Luca Cavallone, Liana Spazzafumo, Francesca Marchegiani, Giuseppina Carrieri, Elena Mugianesi, Claudia Giampieri, Matteo Centurelli, Raffaella Moresi, Silvia Tesei, Rosamaria Lisa, Claudio Viticchi, Lorenzo Falsetti, Stefano Salvioli, Claudio Franceschi

Research output: Contribution to journalArticle

Abstract

In the present investigation we analysed Interleukin 6 (IL-6) in vitro production by Epstein-Barr virus (EBV)-immortalized B lymphocytes established from 43 subjects, 15 young people and 28 elderly people, including 18 centenarians, after 3, 6, 9, 24, 48 and 72 h of culture. The subjects were genotypized for the C to G transition at nucleotide -174 of IL-6 gene promoter (-174 C/G) and were classified as C allele carriers (C+) and non-carriers (C-). We found that: (i) the interindividual difference in in vitro IL-6 production was wider in elderly individuals in respect to young individuals, leading to different coefficient of variation in the two groups; (ii) the -174 C/G polymorphism had an age-related effect on IL-6 in vitro production. Only among C- people, cells from elderly subjects produced significant higher level of IL-6 than cells from young subjects. These data are consistent with our previous results regarding the IL-6 serum levels in a large group of people of different age, including centenarians. Thus, the EBV-immortalized B lymphocytes can be considered a useful in vitro model for studying the genetic control of IL-6 production and its changes with age.

Original languageEnglish
Pages (from-to)549-553
Number of pages5
JournalMechanisms of Ageing and Development
Volume124
Issue number4
DOIs
Publication statusPublished - Apr 1 2003

Keywords

  • Centenarians
  • EBV-immortalized B lymphocytes
  • IL-6 polymorphism

ASJC Scopus subject areas

  • Ageing
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

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    Olivieri, F., Bonafè, M., Giovagnetti, S., Stecconi, R., Cardelli, M., Cavallone, L., Spazzafumo, L., Marchegiani, F., Carrieri, G., Mugianesi, E., Giampieri, C., Centurelli, M., Moresi, R., Tesei, S., Lisa, R., Viticchi, C., Falsetti, L., Salvioli, S., & Franceschi, C. (2003). In vitro IL-6 production by EBV-immortalized B lymphocytes from young and elderly people genotyped for -174 C/G polymorphism in IL-6 gene: A model to study the genetic basis of inflamm-aging. Mechanisms of Ageing and Development, 124(4), 549-553. https://doi.org/10.1016/S0047-6374(03)00035-6