In vitro immune response to rosaprostol (prostaglandinic analogue)

Rosaprostol is a prostaglandin analogue with 18 carbon atoms, structurally related to prostaglandin E (PGE). Since PGE exerts negative effects on immune response, the authors evaluated the possible effects of rosaprostol on in vitro immune response. To this end the mitogen-(PHA)-induced proliferation of peripheral mononuclear cells (PMNCs), PMNC proliferation induced by anti-CD3, anti-CD2 anti-CD28 monoclonal antibodies, and PHA-induced lymphokine (namely interleukin 2 and γ-interferon) secretion were evaluated by adding to cultures 0.05, 0.52 and 5.2 ♂/ml of rosaprostol respectively. The result of the present study demonstrate that rosaprostol does not affect these in votro immunological parameters. For this reason, the structural differences of rosaprostol (18 carbon atoms) play a critical role in the different effects on the immune response, compared with prostaglandins.