In vitro-in vivo fluctuation spectroscopies

M. Collini, L. D'Alfonso, M. Caccia, L. Sironi, M. Panzica, G. Chirico, I. Rivolta, B. Lettiero, G. Miserocchi

Research output: Chapter in Book/Report/Conference proceedingChapter


Fluorescence correlation spectroscopy (FCS) was first developed for biophysical studies in analogy with photon scattering correlation spectroscopy. Although it is mainly devoted to the study of freely diffusing particles, FCS is actually able to discern between different kinds of motions, such as diffusion, anomalous diffusion, or drift motions. The frontier application of FCS nowadays is in medical studies both within cells and on the cell membranes, and in the investigation of single molecules in solid matrices. In this field, FCS originated also image correlation spectroscopy methods. The whole field can be unified under the name of fluorescence fluctuation spectroscopy (FFS). We present here a short review of the theoretical bases of FFS under a unified vision and discuss some applications to the study of dynamics of nanoparticles in cells and to the investigation of the photodynamics of immobilized dyes.

Original languageEnglish
Title of host publicationOptical Fluorescence Microscopy: From the Spectral to the Nano Dimension
PublisherSpringer-Verlag Berlin Heidelberg
Number of pages17
ISBN (Print)9783642151750, 9783642151743
Publication statusPublished - 2011

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)
  • Physics and Astronomy(all)


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