In vitro incubation of bone marrow and peripheral stem cells with vincristine and methylprednisolone: Functional T-cell depletion for haploidentical and autologous transplants

E. Fragonas, S. Perticarari, G. Presani, M. Rabusin, M. Andolina, M. A. Mangiarotti

Research output: Contribution to journalArticle

Abstract

A mismatched bone marrow transplantation is feasible only if the donor's marrow lymphocytes are eliminated from the graft. This can be achieved by several methods, but all have the disadvantage of inducing a long-lasting immune deficiency while the risk of graft rejection and leukemic relapse increase. We use a sort of functional T-cell depletion by treating the cells with vincristine and methylprednisolone. This method is surely the cheapest and has allowed us to perform 60 transplants with a tolerable risk of GVHD. The treatment of the donor's lymphocytes has already been demonstrated to be able to block the mixed lymphocyte culture reaction in vitro. In this experiment Th1 and Th2 activities were almost completely blocked without reduction of lymphocyte viability and apoptosis induction. (C) 2000, Ferrata Storti Foundation.

Original languageEnglish
Pages (from-to)86-88
Number of pages3
JournalHaematologica
Volume85
Issue number11 SUPPL.
Publication statusPublished - 2000

Fingerprint

Methylprednisolone
Autografts
Vincristine
Bone Marrow
Lymphocytes
T-Lymphocytes
Transplants
Mixed Lymphocyte Culture Test
Graft Rejection
Bone Marrow Transplantation
Apoptosis
Recurrence
In Vitro Techniques
Peripheral Blood Stem Cells
Therapeutics

Keywords

  • ABMT
  • Treatment

ASJC Scopus subject areas

  • Hematology

Cite this

In vitro incubation of bone marrow and peripheral stem cells with vincristine and methylprednisolone : Functional T-cell depletion for haploidentical and autologous transplants. / Fragonas, E.; Perticarari, S.; Presani, G.; Rabusin, M.; Andolina, M.; Mangiarotti, M. A.

In: Haematologica, Vol. 85, No. 11 SUPPL., 2000, p. 86-88.

Research output: Contribution to journalArticle

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