In vitro induction of tendon-specific markers in tendon cells, adipose-and bone marrow-derived stem cells is dependent on TGFβ3, BMP-12 and ascorbic acid stimulation

Carlotta Perucca Orfei, Marco Viganò, John R. Pearson, Alessandra Colombini, Paola De Luca, Enrico Ragni, Leonor Santos-Ruiz, Laura de Girolamo

Research output: Contribution to journalArticlepeer-review

Abstract

Mesenchymal Stem Cells (MSCs) and tissue-specific progenitors have been proposed as useful tools for regenerative medicine approaches in bone, cartilage and tendon-related pathologies. The differentiation of cells towards the desired, target tissue-specific lineage has demonstrated advantages in the application of cell therapies and tissue engineering. Unlike osteogenic and chondrogenic differentiation, there is no consensus on the best tenogenic induction protocol. Many growth factors have been proposed for this purpose, including BMP-12, b-FGF, TGF-β3, CTGF, IGF-1 and ascorbic acid (AA). In this study, different combinations of these growth factors have been tested in the context of a two-step differentiation protocol, in order to define their contribution to the induction and maintenance of tendon marker expression in adipose tissue and bone marrow derived MSCs and tendon cells (TCs), respectively. Our results demonstrate that TGF-β3 is the main inducer of scleraxis, an early expressed tendon marker, while at the same time inhibiting tendon markers normally expressed later, such as decorin. In contrast, we find that decorin is induced by BMP-12, b-FGF and AA. Our results provide new insights into the effect of different factors on the tenogenic induction of MSCs and TCs, highlighting the importance of differential timing in TGF-β3 stimulation.

Original languageEnglish
Article number149
JournalInternational Journal of Molecular Sciences
Volume20
Issue number1
DOIs
Publication statusPublished - Jan 1 2019

Keywords

  • Adipose-derived mscs
  • Ascorbic acid
  • Bmp-12
  • Bone marrow-derived mscs
  • Fgf
  • Tendon cells
  • Tenogenic differentiation
  • Tgfbeta

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Fingerprint Dive into the research topics of 'In vitro induction of tendon-specific markers in tendon cells, adipose-and bone marrow-derived stem cells is dependent on TGFβ3, BMP-12 and ascorbic acid stimulation'. Together they form a unique fingerprint.

Cite this