In vitro inhibition of interferon alpha-2a antiproliferative activity by antibodies developed during treatment for essential thrombocythaemia

L. Catani, L. Gugliotta, G. Zauli, G. P. Bagnara, G. Antonelli, M. Mattioli Belmonte, N. Vianelli, L. Bonsi, M. A. Brunelli, S. Tura

Research output: Contribution to journalArticle

Abstract

Background. Various authors have reported the development of anti-interferon (IFN) antibodies following IFN-alpha treatment for haematological malignancies. So far the methods for detecting these antibodies have not considered the antiproliferative activity of this IFN, which is its most important property in anticancer therapy. Methods. In this in vitro study we evaluated the ability of anti-IFN alpha-2a neutralising antibodies to inhibit the antiproliferative activity of IFN alpha-2a and lymphoblastoid IFN alpha using megakaryocyte colony growth as the revelatory system. These antibodies were detected in two patients affected by essential thrombocythaemia (ET) who lost their haematological response to IFN alpha-2a, but responded to a subsequent treatment with lymphoblastoid IFN alpha. Results and Conclusion. The results show that the inhibition of megakaryotype colony growth induced by IFN alpha-2a was totally suppressed in the presence of the two ET patients' sera, whereas the inhibition induced by lymphoblastoid IFN alpha was not significantly affected. These in vitro data demonstrate the high specificity and activity of these antibodies on the antiproliferative effect of IFN alpha-2a.

Original languageEnglish
Pages (from-to)318-321
Number of pages4
JournalHaematologica
Volume77
Issue number4
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Hematology

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    Catani, L., Gugliotta, L., Zauli, G., Bagnara, G. P., Antonelli, G., Mattioli Belmonte, M., Vianelli, N., Bonsi, L., Brunelli, M. A., & Tura, S. (1992). In vitro inhibition of interferon alpha-2a antiproliferative activity by antibodies developed during treatment for essential thrombocythaemia. Haematologica, 77(4), 318-321.