In vitro interaction between lymphocytes and allogeneic leukemic cells

G. Biasi, D. Collavo, A. Colombatti, L. Chieco Bianchi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A study was made of the stimulatory activity of normal spleen versus leukemic cells in culture together with normal allogeneic lymphocytes. The in vitro leukemic cell capacity to generate cytotoxic lymphocytes was investigated as well. The leukemic cells employed apparently did not stimulate allogeneic lymphocytes differing at the H 2 region. Although diffusible inhibitors of cell proliferation and cell surface bound antibody have been reported to interfere with MLR, these factors did not play a significant role. On the other hand, leukemic cells depress normal lymphocyte proliferation, which, however, is not specific for MLR since a strong reduction in 3H TdR uptake was observed also in normal PHA or LPS stimulated cultures. Corollary data suggest that this inhibitory effect is related to the presence of an adherent cell fraction within the leukemic population. Surface antigens coded by genes in the I region of the H 2 complex have been reported to play a prominent role in MLR. These antigens designated as I region associated (Ia) are fully expressed on bone marrow derived (B) lymphocytes, while are absent or weakly present on thymus derived (T) lymphocytes. Studies with lymphoid cell lines derived from leukemic patients have recently demonstrated that B but not T cells possess stimulatory activity in MLR. Typing for T and B cell markers showed that, with the exception of LSTRA, a BALB/c substrain, which is considered monocytoid in origin, all leukemias employed in the present study are T cell derived. The possibility that the failure to produce MLR is therefore linked to the T cell characteristic of the leukemic lines should be considered, just as is the case for the Ia antigens.

Original languageEnglish
Title of host publicationBibliotheca Haematologica
Pages34-36
Number of pages3
VolumeNo.43
Publication statusPublished - 1976

ASJC Scopus subject areas

  • Hematology

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