A study was undertaken to identify an in vitro model to compare the efficacy of decontamination procedures for infected catheters. Colonization of the catheter was achieved by inoculating the catheter with an high number of bacteria (1 liter of broth containing 150 x 106 CFU/ml per diem x 3 days). After contamination, the catheter was cut in 10 segments. Each segment was placed in a simple sterile container at constant temperature (37°C) and connected to an infusion device. Clindamycin, vancomycin and Imipenem were challenged at three low dosage levels against 20 clinically isolated strains of Staphylococcus spp. Drugs were continuously administered into the contaminated catheter segments for a period of five hours. Regular counts of viable bacteria in the effluent compared to the counts derived from a non-treated segment were carried out. Results suggest that this in vitro model can be a useful tool to study dosage schedules to prevent catheter contamination or to treat catheter related sepsis with least side effects for patients and on host-associated non pathogenic bacterial microflora.
|Translated title of the contribution||In vitro model to study the activity of antibiotics on bacteria attached to central venous catheter|
|Number of pages||5|
|Journal||Giornale di Malattie Infettive e Parassitarie|
|Publication status||Published - 1994|
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