TY - JOUR
T1 - In vitro modulation of gamma amino butyric acid (GABA) receptor expression by bone marrow stromal cells
AU - Marcoli, Manuela
AU - Candiani, Simona
AU - Tonachini, Laura
AU - Monticone, Massimiliano
AU - Mastrogiacomo, Maddalena
AU - Ottonello, Andrea
AU - Cervetto, Chiara
AU - Paluzzi, Paola
AU - Maura, Guido
AU - Pestarino, Mario
AU - Cancedda, Ranieri
AU - Castagnola, Patrizio
PY - 2008/5
Y1 - 2008/5
N2 - Bone marrow stromal cells (BMSC) have the capability to undergo a change of morphology, reminiscent of neuronal cells, after exposure to an inductive medium. These induced BMSC-derived neuron-like (BDNL) cells express several neuronal markers, including Microtubule-Associated Protein Tau, Neurofilament M, and Nestin as revealed by immunocytochemistry analysis. To assess whether the induction process has possible functional relevance, we have focused our attention on the expression of neurotransmitter receptors. In particular, we show that the expression of GABAA subunits α1, β2/3, ε and GABAB1 mRNAs is greatly enhanced in BMSC by the induction treatment. Similar results were obtained from rat skin fibroblasts subjected to the same induction protocol, with the exception for the GABAB2 transcript that was expressed only by BMSC and BDNL. The presence of both GABAB1 and GABAB2 subunits in BDNL cells suggests that functional GABAB receptors might be assembled: we indeed found that a functional GABAB receptor, negatively linked to cyclic AMP production, is expressed in BDNL. Therefore, we suggest that BMSC can be converted into cells equipped with appropriate receptors coupled to transduction mechanisms, potentially responding to a specific neurotransmitter.
AB - Bone marrow stromal cells (BMSC) have the capability to undergo a change of morphology, reminiscent of neuronal cells, after exposure to an inductive medium. These induced BMSC-derived neuron-like (BDNL) cells express several neuronal markers, including Microtubule-Associated Protein Tau, Neurofilament M, and Nestin as revealed by immunocytochemistry analysis. To assess whether the induction process has possible functional relevance, we have focused our attention on the expression of neurotransmitter receptors. In particular, we show that the expression of GABAA subunits α1, β2/3, ε and GABAB1 mRNAs is greatly enhanced in BMSC by the induction treatment. Similar results were obtained from rat skin fibroblasts subjected to the same induction protocol, with the exception for the GABAB2 transcript that was expressed only by BMSC and BDNL. The presence of both GABAB1 and GABAB2 subunits in BDNL cells suggests that functional GABAB receptors might be assembled: we indeed found that a functional GABAB receptor, negatively linked to cyclic AMP production, is expressed in BDNL. Therefore, we suggest that BMSC can be converted into cells equipped with appropriate receptors coupled to transduction mechanisms, potentially responding to a specific neurotransmitter.
KW - Bone marrow stromal cells
KW - GABA receptors
KW - Gene expression
KW - Pharmacological assay
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U2 - 10.1016/j.phrs.2008.03.012
DO - 10.1016/j.phrs.2008.03.012
M3 - Article
C2 - 18467116
AN - SCOPUS:44549083266
VL - 57
SP - 374
EP - 382
JO - Pharmacological Research
JF - Pharmacological Research
SN - 1043-6618
IS - 5
ER -