In vitro osteoblastic differentiation of human mesenchymal stem cells and human dental pulp stem cells on poly- L -lysine-treated titanium-6-aluminium-4- vanadium

Daniela Galli, Laura Benedetti, Matilde Bongio, Valentina Maliardi, Giulia Silvani, Gabriele Ceccarelli, Flavio Ronzoni, Silvio Conte, Francesco Benazzo, Antonio Graziano, Gianpaolo Papaccio, Maurilio Sampaolesi, Maria Gabriella Cusella De Angelis

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Three-dimensional (3D) titanium-6-aluminium-4-vanadium (Ti6Al4V) is a widely used biomaterial for orthopedic prosthesis and dental implants; thanks to its very high-mechanical strength and resistance to corrosion. Human mesenchymal stem cells (hMSCs) and dental pulp stem cells (hDPSCs) are responsible for bone regeneration following colonization of prosthesis or dental implants. Both hMSCs and hDPSCs have lower ability to colonize this biomaterial in comparison with tissue culture-treated plastic. Both hMSCs and hDPSCs show lack of focal adhesion kinase (FAK) activation when grown on Ti6Al4V. This signal is restored in the presence of poly-L-lysine (poly-L-lys). Poly-L-lys has been used as part of organoapatite or together with zinc and calcium ions. Our results suggest that poly-L-lys alone induces FAK activation through β1-INTEGRIN, because the presence of β1-INTEGRIN blocking antibody avoided FAK autophosphorylation. Presence of poly-L-lys also increases expression of osteoblastic differentiation marker genes in hMSCs and hDPSCs grown on Ti6Al4V. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.

Original languageEnglish
Pages (from-to)118-126
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume97 A
Issue number2
DOIs
Publication statusPublished - May 2011

Fingerprint

Dental Pulp
Stem cells
Mesenchymal Stromal Cells
Vanadium
Focal Adhesion Protein-Tyrosine Kinases
Lysine
Pulp
Stem Cells
Titanium
Aluminum
Dental Implants
Biocompatible Materials
Prostheses and Implants
Dental prostheses
Bone Regeneration
Blocking Antibodies
Corrosion
Adhesion
Differentiation Antigens
Biomaterials

Keywords

  • FAK
  • osteoblastic differentiation
  • poly- L -lysine
  • Ti6Al4V

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys

Cite this

In vitro osteoblastic differentiation of human mesenchymal stem cells and human dental pulp stem cells on poly- L -lysine-treated titanium-6-aluminium-4- vanadium. / Galli, Daniela; Benedetti, Laura; Bongio, Matilde; Maliardi, Valentina; Silvani, Giulia; Ceccarelli, Gabriele; Ronzoni, Flavio; Conte, Silvio; Benazzo, Francesco; Graziano, Antonio; Papaccio, Gianpaolo; Sampaolesi, Maurilio; Cusella De Angelis, Maria Gabriella.

In: Journal of Biomedical Materials Research - Part A, Vol. 97 A, No. 2, 05.2011, p. 118-126.

Research output: Contribution to journalArticle

Galli, D, Benedetti, L, Bongio, M, Maliardi, V, Silvani, G, Ceccarelli, G, Ronzoni, F, Conte, S, Benazzo, F, Graziano, A, Papaccio, G, Sampaolesi, M & Cusella De Angelis, MG 2011, 'In vitro osteoblastic differentiation of human mesenchymal stem cells and human dental pulp stem cells on poly- L -lysine-treated titanium-6-aluminium-4- vanadium', Journal of Biomedical Materials Research - Part A, vol. 97 A, no. 2, pp. 118-126. https://doi.org/10.1002/jbm.a.32996
Galli, Daniela ; Benedetti, Laura ; Bongio, Matilde ; Maliardi, Valentina ; Silvani, Giulia ; Ceccarelli, Gabriele ; Ronzoni, Flavio ; Conte, Silvio ; Benazzo, Francesco ; Graziano, Antonio ; Papaccio, Gianpaolo ; Sampaolesi, Maurilio ; Cusella De Angelis, Maria Gabriella. / In vitro osteoblastic differentiation of human mesenchymal stem cells and human dental pulp stem cells on poly- L -lysine-treated titanium-6-aluminium-4- vanadium. In: Journal of Biomedical Materials Research - Part A. 2011 ; Vol. 97 A, No. 2. pp. 118-126.
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