Abstract
Three-dimensional (3D) titanium-6-aluminium-4-vanadium (Ti6Al4V) is a widely used biomaterial for orthopedic prosthesis and dental implants; thanks to its very high-mechanical strength and resistance to corrosion. Human mesenchymal stem cells (hMSCs) and dental pulp stem cells (hDPSCs) are responsible for bone regeneration following colonization of prosthesis or dental implants. Both hMSCs and hDPSCs have lower ability to colonize this biomaterial in comparison with tissue culture-treated plastic. Both hMSCs and hDPSCs show lack of focal adhesion kinase (FAK) activation when grown on Ti6Al4V. This signal is restored in the presence of poly-L-lysine (poly-L-lys). Poly-L-lys has been used as part of organoapatite or together with zinc and calcium ions. Our results suggest that poly-L-lys alone induces FAK activation through β1-INTEGRIN, because the presence of β1-INTEGRIN blocking antibody avoided FAK autophosphorylation. Presence of poly-L-lys also increases expression of osteoblastic differentiation marker genes in hMSCs and hDPSCs grown on Ti6Al4V. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
| Original language | English |
|---|---|
| Pages (from-to) | 118-126 |
| Number of pages | 9 |
| Journal | Journal of Biomedical Materials Research - Part A |
| Volume | 97 A |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - May 2011 |
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Keywords
- FAK
- osteoblastic differentiation
- poly- L -lysine
- Ti6Al4V
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials
- Ceramics and Composites
- Metals and Alloys
Cite this
In vitro osteoblastic differentiation of human mesenchymal stem cells and human dental pulp stem cells on poly- L -lysine-treated titanium-6-aluminium-4- vanadium. / Galli, Daniela; Benedetti, Laura; Bongio, Matilde; Maliardi, Valentina; Silvani, Giulia; Ceccarelli, Gabriele; Ronzoni, Flavio; Conte, Silvio; Benazzo, Francesco; Graziano, Antonio; Papaccio, Gianpaolo; Sampaolesi, Maurilio; Cusella De Angelis, Maria Gabriella.
In: Journal of Biomedical Materials Research - Part A, Vol. 97 A, No. 2, 05.2011, p. 118-126.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro osteoblastic differentiation of human mesenchymal stem cells and human dental pulp stem cells on poly- L -lysine-treated titanium-6-aluminium-4- vanadium
AU - Galli, Daniela
AU - Benedetti, Laura
AU - Bongio, Matilde
AU - Maliardi, Valentina
AU - Silvani, Giulia
AU - Ceccarelli, Gabriele
AU - Ronzoni, Flavio
AU - Conte, Silvio
AU - Benazzo, Francesco
AU - Graziano, Antonio
AU - Papaccio, Gianpaolo
AU - Sampaolesi, Maurilio
AU - Cusella De Angelis, Maria Gabriella
PY - 2011/5
Y1 - 2011/5
N2 - Three-dimensional (3D) titanium-6-aluminium-4-vanadium (Ti6Al4V) is a widely used biomaterial for orthopedic prosthesis and dental implants; thanks to its very high-mechanical strength and resistance to corrosion. Human mesenchymal stem cells (hMSCs) and dental pulp stem cells (hDPSCs) are responsible for bone regeneration following colonization of prosthesis or dental implants. Both hMSCs and hDPSCs have lower ability to colonize this biomaterial in comparison with tissue culture-treated plastic. Both hMSCs and hDPSCs show lack of focal adhesion kinase (FAK) activation when grown on Ti6Al4V. This signal is restored in the presence of poly-L-lysine (poly-L-lys). Poly-L-lys has been used as part of organoapatite or together with zinc and calcium ions. Our results suggest that poly-L-lys alone induces FAK activation through β1-INTEGRIN, because the presence of β1-INTEGRIN blocking antibody avoided FAK autophosphorylation. Presence of poly-L-lys also increases expression of osteoblastic differentiation marker genes in hMSCs and hDPSCs grown on Ti6Al4V. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
AB - Three-dimensional (3D) titanium-6-aluminium-4-vanadium (Ti6Al4V) is a widely used biomaterial for orthopedic prosthesis and dental implants; thanks to its very high-mechanical strength and resistance to corrosion. Human mesenchymal stem cells (hMSCs) and dental pulp stem cells (hDPSCs) are responsible for bone regeneration following colonization of prosthesis or dental implants. Both hMSCs and hDPSCs have lower ability to colonize this biomaterial in comparison with tissue culture-treated plastic. Both hMSCs and hDPSCs show lack of focal adhesion kinase (FAK) activation when grown on Ti6Al4V. This signal is restored in the presence of poly-L-lysine (poly-L-lys). Poly-L-lys has been used as part of organoapatite or together with zinc and calcium ions. Our results suggest that poly-L-lys alone induces FAK activation through β1-INTEGRIN, because the presence of β1-INTEGRIN blocking antibody avoided FAK autophosphorylation. Presence of poly-L-lys also increases expression of osteoblastic differentiation marker genes in hMSCs and hDPSCs grown on Ti6Al4V. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
KW - FAK
KW - osteoblastic differentiation
KW - poly- L -lysine
KW - Ti6Al4V
UR - http://www.scopus.com/inward/record.url?scp=79953035928&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79953035928&partnerID=8YFLogxK
U2 - 10.1002/jbm.a.32996
DO - 10.1002/jbm.a.32996
M3 - Article
C2 - 21370441
AN - SCOPUS:79953035928
VL - 97 A
SP - 118
EP - 126
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
SN - 1549-3296
IS - 2
ER -