Abstract
Tissue engineering (by culturing cells on appropriate scaffolds, and using bioreactors to drive the correct bone structure formation) is an attractive alternative to bone grafting or implantation of bone substitutes. Osteogenesis is a biological process that involves many molecular intracellular pathways organized to optimize bone modeling. The use of bioreactor systems and especially the perfusion bioreactor, provides both the technological means to reveal fundamental mechanisms of cell function in a 3D environment, and the potential to improve the quality of engineered tissues. In this mini-review all the characteristics for the production of an appropriate bone construct are analyzed: the stem cell source, scaffolds useful for the seeding of pre-osteoblastic cells and the effects of fluid flow on differentiation and proliferation of bone precursor cells. By automating and standardizing tissue manufacture in controlled closed systems, engineered tissues may reduce the gap between the process of bone formation in vitro and subsequent graft of bone substitutes in vivo.
| Original language | English |
|---|---|
| Pages (from-to) | 29-38 |
| Number of pages | 10 |
| Journal | Recent Patents on Drug Delivery and Formulation |
| Volume | 7 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2013 |
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Keywords
- Biomaterials
- Extracellular matrix
- Induced pluripotent stem cells (iPSCs)
- Mesenchymal stem cells (MSC)
- Os-teogenesis
- Perfusion bioreactor
- Scaffolds
- Tissue engineering
ASJC Scopus subject areas
- Pharmaceutical Science
- Biomedical Engineering
Cite this
In vitro osteogenesis of human stem cells by using a three-dimensional perfusion bioreactor culture system : A review. / Ceccarelli, Gabriele; Bloise, Nora; Vercellino, Marco; Battaglia, Rosalia; Morgante, Lucia; de Angelis, Maria Gabriella Cusella; Imbriani, Marcello; Visai, Livia.
In: Recent Patents on Drug Delivery and Formulation, Vol. 7, No. 1, 2013, p. 29-38.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro osteogenesis of human stem cells by using a three-dimensional perfusion bioreactor culture system
T2 - A review
AU - Ceccarelli, Gabriele
AU - Bloise, Nora
AU - Vercellino, Marco
AU - Battaglia, Rosalia
AU - Morgante, Lucia
AU - de Angelis, Maria Gabriella Cusella
AU - Imbriani, Marcello
AU - Visai, Livia
PY - 2013
Y1 - 2013
N2 - Tissue engineering (by culturing cells on appropriate scaffolds, and using bioreactors to drive the correct bone structure formation) is an attractive alternative to bone grafting or implantation of bone substitutes. Osteogenesis is a biological process that involves many molecular intracellular pathways organized to optimize bone modeling. The use of bioreactor systems and especially the perfusion bioreactor, provides both the technological means to reveal fundamental mechanisms of cell function in a 3D environment, and the potential to improve the quality of engineered tissues. In this mini-review all the characteristics for the production of an appropriate bone construct are analyzed: the stem cell source, scaffolds useful for the seeding of pre-osteoblastic cells and the effects of fluid flow on differentiation and proliferation of bone precursor cells. By automating and standardizing tissue manufacture in controlled closed systems, engineered tissues may reduce the gap between the process of bone formation in vitro and subsequent graft of bone substitutes in vivo.
AB - Tissue engineering (by culturing cells on appropriate scaffolds, and using bioreactors to drive the correct bone structure formation) is an attractive alternative to bone grafting or implantation of bone substitutes. Osteogenesis is a biological process that involves many molecular intracellular pathways organized to optimize bone modeling. The use of bioreactor systems and especially the perfusion bioreactor, provides both the technological means to reveal fundamental mechanisms of cell function in a 3D environment, and the potential to improve the quality of engineered tissues. In this mini-review all the characteristics for the production of an appropriate bone construct are analyzed: the stem cell source, scaffolds useful for the seeding of pre-osteoblastic cells and the effects of fluid flow on differentiation and proliferation of bone precursor cells. By automating and standardizing tissue manufacture in controlled closed systems, engineered tissues may reduce the gap between the process of bone formation in vitro and subsequent graft of bone substitutes in vivo.
KW - Biomaterials
KW - Extracellular matrix
KW - Induced pluripotent stem cells (iPSCs)
KW - Mesenchymal stem cells (MSC)
KW - Os-teogenesis
KW - Perfusion bioreactor
KW - Scaffolds
KW - Tissue engineering
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UR - http://www.scopus.com/inward/citedby.url?scp=84873177367&partnerID=8YFLogxK
U2 - 10.2174/187221113804805801
DO - 10.2174/187221113804805801
M3 - Article
VL - 7
SP - 29
EP - 38
JO - Recent Patents on Drug Delivery and Formulation
JF - Recent Patents on Drug Delivery and Formulation
SN - 1872-2113
IS - 1
ER -