In vitro osteogenesis of human stem cells by using a three-dimensional perfusion bioreactor culture system

A review

Gabriele Ceccarelli, Nora Bloise, Marco Vercellino, Rosalia Battaglia, Lucia Morgante, Maria Gabriella Cusella de Angelis, Marcello Imbriani, Livia Visai

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Tissue engineering (by culturing cells on appropriate scaffolds, and using bioreactors to drive the correct bone structure formation) is an attractive alternative to bone grafting or implantation of bone substitutes. Osteogenesis is a biological process that involves many molecular intracellular pathways organized to optimize bone modeling. The use of bioreactor systems and especially the perfusion bioreactor, provides both the technological means to reveal fundamental mechanisms of cell function in a 3D environment, and the potential to improve the quality of engineered tissues. In this mini-review all the characteristics for the production of an appropriate bone construct are analyzed: the stem cell source, scaffolds useful for the seeding of pre-osteoblastic cells and the effects of fluid flow on differentiation and proliferation of bone precursor cells. By automating and standardizing tissue manufacture in controlled closed systems, engineered tissues may reduce the gap between the process of bone formation in vitro and subsequent graft of bone substitutes in vivo.

Original languageEnglish
Pages (from-to)29-38
Number of pages10
JournalRecent Patents on Drug Delivery and Formulation
Volume7
Issue number1
DOIs
Publication statusPublished - 2013

Fingerprint

Bioreactors
Stem cells
Osteogenesis
Bone
Stem Cells
Perfusion
Bone Substitutes
Bone and Bones
Biological Phenomena
Bone Transplantation
Tissue Engineering
Tissue
Transplants
In Vitro Techniques
Scaffolds (biology)
Tissue engineering
Grafts
Scaffolds
Ion implantation
Flow of fluids

Keywords

  • Biomaterials
  • Extracellular matrix
  • Induced pluripotent stem cells (iPSCs)
  • Mesenchymal stem cells (MSC)
  • Os-teogenesis
  • Perfusion bioreactor
  • Scaffolds
  • Tissue engineering

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Biomedical Engineering

Cite this

In vitro osteogenesis of human stem cells by using a three-dimensional perfusion bioreactor culture system : A review. / Ceccarelli, Gabriele; Bloise, Nora; Vercellino, Marco; Battaglia, Rosalia; Morgante, Lucia; de Angelis, Maria Gabriella Cusella; Imbriani, Marcello; Visai, Livia.

In: Recent Patents on Drug Delivery and Formulation, Vol. 7, No. 1, 2013, p. 29-38.

Research output: Contribution to journalArticle

Ceccarelli, Gabriele ; Bloise, Nora ; Vercellino, Marco ; Battaglia, Rosalia ; Morgante, Lucia ; de Angelis, Maria Gabriella Cusella ; Imbriani, Marcello ; Visai, Livia. / In vitro osteogenesis of human stem cells by using a three-dimensional perfusion bioreactor culture system : A review. In: Recent Patents on Drug Delivery and Formulation. 2013 ; Vol. 7, No. 1. pp. 29-38.
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