In vitro oxygenation of soybean biomembranes by lipoxygenase-2

Mauro Maccarrone, Peter G M van Aarle, Gerrit A. Veldink, Johannes F G Vliegenthart

Research output: Contribution to journalArticle

Abstract

The ability of soybean lipoxygenases-1 and -2 to oxygenate biomembranes isolated from soybean seedlings has been investigated. Constituents of the lipid bilayer were analyzed by means of reversed phase and chiral phase high performance liquid chromatography, gas chromatography/mass spectrometry, high performance thin layer chromatography and uv spectroscopy. Evidence is presented that soybean lipoxygenase-2, at variance with the type-1 enzyme, oxygenates the esterified unsaturated fatty acid moieties in biomembranes, whereas membrane-embedded free unsaturated fatty acid moieties were not a suitable substrate for either isoenzyme. The oxygenation products derived from the biomembranes were the 9- and 13-hydroperoxides of linoleic acid residues, in a molar ratio of 1.0 to 1.7, and the 9- and 13-hydroperoxides of α-linolenic acid residues, in a molar ratio of 1.0 to 0.1. The R S ratios of 13-hydroperoxy-9Z,11E-octadecadienoic acid and 9-hydroperoxy-10E,12Z,15Z-octadecatrienoic acid were found to be 0.5 and 25.0, respectively. These stereospecificity values were much higher than those of hydroperoxides isolated after incubation of lipoxygenase-2 with non-membraneous fatty acids or their methyl esters. The hydroperoxy fatty acids produced were distributed in neutral lipids and phospholipids isolated from soybean membranes, the former being oxidized to a larger extent. Furthermore, both intracellular and plasma membranes were substrates for the enzymic oxygenation, with a preference for those of chloroplasts followed by those of Golgi apparatus, endoplasmic reticulum, plasma membrane and mitochondria. These data point towards a different action of the two lipoxygenases in soybean cells. We suggest that the type-2 enzyme plays a role in the in vivo remodelling of biomembranes. The physiological relevance of these findings is discussed.

Original languageEnglish
Pages (from-to)164-169
Number of pages6
JournalBBA - Biomembranes
Volume1190
Issue number1
DOIs
Publication statusPublished - Feb 23 1994

Keywords

  • (G. max)
  • Biomembrane
  • Lipoxygenase-1
  • Lipoxygenase-2

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

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  • Cite this

    Maccarrone, M., van Aarle, P. G. M., Veldink, G. A., & Vliegenthart, J. F. G. (1994). In vitro oxygenation of soybean biomembranes by lipoxygenase-2. BBA - Biomembranes, 1190(1), 164-169. https://doi.org/10.1016/0005-2736(94)90046-9