In vitro preclinical models for a rational design of chemotherapy combinations in human tumors

Wainer Zoli, Luca Ricotti, Anna Tesei, Fabio Barzanti, Dino Amadori

Research output: Contribution to journalArticle

Abstract

Today, drug combinations are frequently used in the treatment of cancer to increase therapeutic efficacy. Currently used clinical protocols for cancer combination therapies are mainly obtained empirically or on the basis of results from previous clinical trials. Information obtained from clinical protocols is invaluable, but it is time-consuming, expensive and does not provide data on the biochemical and molecular mechanisms of interaction of the drugs used in combination treatments at cellular level. Therefore, in vitro drug combination studies on established cell lines or primary cell cultures play an important role in designing and optimising combination protocols. A variety of in vitro assays and different mathematics models have been developed to investigate cytotoxic effects and to analyse the type of drug interactions. Increased knowledge of the cellular targets of traditional and new drugs and the development of new technologies have resulted in a new role for the in vitro tests which are no longer used only to evaluate the cytotoxic effects of drugs, but also to investigate the interference on cell cycle, induction of apoptosis and molecular or biochemical interactions. A review on in vitro preclinical tests used to evaluate the effects of drug combinations and to design the rationale of combined chemotherapy protocols is presented.

Original languageEnglish
Pages (from-to)69-82
Number of pages14
JournalCritical Reviews in Oncology/Hematology
Volume37
Issue number1
DOIs
Publication statusPublished - 2001

Keywords

  • Cell lines
  • Chemotherapy combinations
  • In vitro models
  • Preclinical study

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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