In vitro production of a human HLA alloantibody of restricted specificity (DQw2) via Epstein-Barr virus transformation

Maria Pia Pistillo; Ulrich Hämmerling; Bo Dupont; Giovan Battista Ferrara

doi:10.1016/0198-8859(86)90321-6

In vitro production of a human HLA alloantibody of restricted specificity (DQw2) via Epstein-Barr virus transformation

Maria Pia Pistillo, Ulrich Hämmerling, Bo Dupont, Giovan Battista Ferrara

A.O.U. San Martino - IST, Istituto Nazionale Ricerca sul Cancro (GENOVA)

Research output: Contribution to journal › Article › peer-review

Abstract

This paper describes the in vitro production of the human alloantibody 454A.C5 with HLA-DQw2 specificity produced by a stable EBV-transformed cell line. Immune B cells for transformation were generated by planned immunization of a blood donor volunteer with repeated HLA-immunizations over the course of 9 yr. Reactivity of 454A.C5 was in exact concordance with the presence of the DQw2 antigen as defined by microcytotoxicity assay on a panel of 21 B lymphoblastoid cell lines with well-characterized HLA antigens. Family studies showed the segregation according to the HLA-DQw2 haplotype; thus we demonstrate by serological and formal genetics criteria, that the alloantibody is specifically directed against the DQw2. 454A.C5 antibody may be employed as a monospecific HLA typing reagent.

Original language	English
Pages (from-to)	109-117
Number of pages	9
Journal	Human Immunology
Volume	15
Issue number	1
DOIs	https://doi.org/10.1016/0198-8859(86)90321-6
Publication status	Published - 1986

ASJC Scopus subject areas

Immunology
Immunology and Allergy

Access to Document

10.1016/0198-8859(86)90321-6

Cite this

@article{2793246de37d48dbbe25a5f380226aaa,

title = "In vitro production of a human HLA alloantibody of restricted specificity (DQw2) via Epstein-Barr virus transformation",

abstract = "This paper describes the in vitro production of the human alloantibody 454A.C5 with HLA-DQw2 specificity produced by a stable EBV-transformed cell line. Immune B cells for transformation were generated by planned immunization of a blood donor volunteer with repeated HLA-immunizations over the course of 9 yr. Reactivity of 454A.C5 was in exact concordance with the presence of the DQw2 antigen as defined by microcytotoxicity assay on a panel of 21 B lymphoblastoid cell lines with well-characterized HLA antigens. Family studies showed the segregation according to the HLA-DQw2 haplotype; thus we demonstrate by serological and formal genetics criteria, that the alloantibody is specifically directed against the DQw2. 454A.C5 antibody may be employed as a monospecific HLA typing reagent.",

author = "{Pia Pistillo}, Maria and Ulrich H{\"a}mmerling and Bo Dupont and {Battista Ferrara}, Giovan",

year = "1986",

doi = "10.1016/0198-8859(86)90321-6",

language = "English",

volume = "15",

pages = "109--117",

journal = "Human Immunology",

issn = "0198-8859",

publisher = "Elsevier Inc.",

number = "1",

}

TY - JOUR

T1 - In vitro production of a human HLA alloantibody of restricted specificity (DQw2) via Epstein-Barr virus transformation

AU - Pia Pistillo, Maria

AU - Hämmerling, Ulrich

AU - Dupont, Bo

AU - Battista Ferrara, Giovan

PY - 1986

Y1 - 1986

N2 - This paper describes the in vitro production of the human alloantibody 454A.C5 with HLA-DQw2 specificity produced by a stable EBV-transformed cell line. Immune B cells for transformation were generated by planned immunization of a blood donor volunteer with repeated HLA-immunizations over the course of 9 yr. Reactivity of 454A.C5 was in exact concordance with the presence of the DQw2 antigen as defined by microcytotoxicity assay on a panel of 21 B lymphoblastoid cell lines with well-characterized HLA antigens. Family studies showed the segregation according to the HLA-DQw2 haplotype; thus we demonstrate by serological and formal genetics criteria, that the alloantibody is specifically directed against the DQw2. 454A.C5 antibody may be employed as a monospecific HLA typing reagent.

AB - This paper describes the in vitro production of the human alloantibody 454A.C5 with HLA-DQw2 specificity produced by a stable EBV-transformed cell line. Immune B cells for transformation were generated by planned immunization of a blood donor volunteer with repeated HLA-immunizations over the course of 9 yr. Reactivity of 454A.C5 was in exact concordance with the presence of the DQw2 antigen as defined by microcytotoxicity assay on a panel of 21 B lymphoblastoid cell lines with well-characterized HLA antigens. Family studies showed the segregation according to the HLA-DQw2 haplotype; thus we demonstrate by serological and formal genetics criteria, that the alloantibody is specifically directed against the DQw2. 454A.C5 antibody may be employed as a monospecific HLA typing reagent.

UR - http://www.scopus.com/inward/record.url?scp=0022629818&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022629818&partnerID=8YFLogxK

U2 - 10.1016/0198-8859(86)90321-6

DO - 10.1016/0198-8859(86)90321-6

M3 - Article

C2 - 2419282

AN - SCOPUS:0022629818

VL - 15

SP - 109

EP - 117

JO - Human Immunology

JF - Human Immunology

SN - 0198-8859

IS - 1

ER -

In vitro production of a human HLA alloantibody of restricted specificity (DQw2) via Epstein-Barr virus transformation

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this