In vitro production of a human HLA alloantibody of restricted specificity (DQw2) via Epstein-Barr virus transformation

Maria Pia Pistillo; Ulrich Hämmerling; Bo Dupont; Giovan Battista Ferrara

doi:10.1016/0198-8859(86)90321-6

In vitro production of a human HLA alloantibody of restricted specificity (DQw2) via Epstein-Barr virus transformation

Maria Pia Pistillo, Ulrich Hämmerling, Bo Dupont, Giovan Battista Ferrara

A.O.U. San Martino - IST, Istituto Nazionale Ricerca sul Cancro (GENOVA)

Research output: Contribution to journal › Article › peer-review

Abstract

This paper describes the in vitro production of the human alloantibody 454A.C5 with HLA-DQw2 specificity produced by a stable EBV-transformed cell line. Immune B cells for transformation were generated by planned immunization of a blood donor volunteer with repeated HLA-immunizations over the course of 9 yr. Reactivity of 454A.C5 was in exact concordance with the presence of the DQw2 antigen as defined by microcytotoxicity assay on a panel of 21 B lymphoblastoid cell lines with well-characterized HLA antigens. Family studies showed the segregation according to the HLA-DQw2 haplotype; thus we demonstrate by serological and formal genetics criteria, that the alloantibody is specifically directed against the DQw2. 454A.C5 antibody may be employed as a monospecific HLA typing reagent.

Original language	English
Pages (from-to)	109-117
Number of pages	9
Journal	Human Immunology
Volume	15
Issue number	1
DOIs	https://doi.org/10.1016/0198-8859(86)90321-6
Publication status	Published - 1986

ASJC Scopus subject areas

Immunology
Immunology and Allergy

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title = "In vitro production of a human HLA alloantibody of restricted specificity (DQw2) via Epstein-Barr virus transformation",

abstract = "This paper describes the in vitro production of the human alloantibody 454A.C5 with HLA-DQw2 specificity produced by a stable EBV-transformed cell line. Immune B cells for transformation were generated by planned immunization of a blood donor volunteer with repeated HLA-immunizations over the course of 9 yr. Reactivity of 454A.C5 was in exact concordance with the presence of the DQw2 antigen as defined by microcytotoxicity assay on a panel of 21 B lymphoblastoid cell lines with well-characterized HLA antigens. Family studies showed the segregation according to the HLA-DQw2 haplotype; thus we demonstrate by serological and formal genetics criteria, that the alloantibody is specifically directed against the DQw2. 454A.C5 antibody may be employed as a monospecific HLA typing reagent.",

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T1 - In vitro production of a human HLA alloantibody of restricted specificity (DQw2) via Epstein-Barr virus transformation

AU - Pia Pistillo, Maria

AU - Hämmerling, Ulrich

AU - Dupont, Bo

AU - Battista Ferrara, Giovan

PY - 1986

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AB - This paper describes the in vitro production of the human alloantibody 454A.C5 with HLA-DQw2 specificity produced by a stable EBV-transformed cell line. Immune B cells for transformation were generated by planned immunization of a blood donor volunteer with repeated HLA-immunizations over the course of 9 yr. Reactivity of 454A.C5 was in exact concordance with the presence of the DQw2 antigen as defined by microcytotoxicity assay on a panel of 21 B lymphoblastoid cell lines with well-characterized HLA antigens. Family studies showed the segregation according to the HLA-DQw2 haplotype; thus we demonstrate by serological and formal genetics criteria, that the alloantibody is specifically directed against the DQw2. 454A.C5 antibody may be employed as a monospecific HLA typing reagent.

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