In vitro production of human anti-sperm antibodies: effect of an oligoclonal antibody (F6) on sperm-egg interaction

F. M. Fusi, F. Besuschio, L. De Santis, I. Lorenzetti, A. Ferrari

Research output: Contribution to journalArticle

Abstract

A method has been developed to establish lines of transformed lymphocytes able to produce in vitro the same anti-sperm antibodies as those naturally occurring in immuno-infertile individuals. We utilized lymphocytes from a male donor whose serum contained anti-sperm antibodies of the IgG class up to the dilution 1:10 000, as detected by means of immunobead binding. T lymphocytes were separated from B lymphocytes using magnetic beads coated with anti-T antibody. B lymphocytes were then placed at a concentration of 5 × 106/ml in a 96-well plate, stimulated with phytohaemagglutinin (PHA) and transformed with Epstein-Barr virus. After a few days, only transformed cells continued growing and these were collected. The supernatant was tested for production of anti-sperm antibodies and those transformed lymphocytes shown to be synthesising antibodies directed against the sperm head and the tail were cloned. We obtained a clone of cells producing antibodies of the IgG1 class directed against the head of the spermatozoon. This oligoclonal antibody (F6) recognized a 58-kDa band from a lysate of sperm membranes and was able to reduce the penetration of zona-free hamster oocytes by capacitated spermatozoa.

Original languageEnglish
Pages (from-to)135-147
Number of pages13
JournalJournal of Reproductive Immunology
Volume29
Issue number2
DOIs
Publication statusPublished - 1995

Fingerprint

Sperm-Ovum Interactions
Spermatozoa
Anti-Idiotypic Antibodies
Antibodies
Immunoglobulin Isotypes
Lymphocytes
B-Lymphocytes
Immunoglobulin G
Sperm Tail
Sperm Head
Herpes Zoster
Phytohemagglutinins
Human Herpesvirus 4
Cricetinae
Oocytes
In Vitro Techniques
Clone Cells
T-Lymphocytes
Membranes
Serum

Keywords

  • Human anti-sperm antibodies
  • In vitro production
  • Inhibition of sperm-egg interaction

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

In vitro production of human anti-sperm antibodies : effect of an oligoclonal antibody (F6) on sperm-egg interaction. / Fusi, F. M.; Besuschio, F.; De Santis, L.; Lorenzetti, I.; Ferrari, A.

In: Journal of Reproductive Immunology, Vol. 29, No. 2, 1995, p. 135-147.

Research output: Contribution to journalArticle

Fusi, F. M. ; Besuschio, F. ; De Santis, L. ; Lorenzetti, I. ; Ferrari, A. / In vitro production of human anti-sperm antibodies : effect of an oligoclonal antibody (F6) on sperm-egg interaction. In: Journal of Reproductive Immunology. 1995 ; Vol. 29, No. 2. pp. 135-147.
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