In vitro production of tumor necrosis factor-α, interleukin-6 and interleukin-8 from normal human peripheral blood mononuclear cells stimulated by Rhodococcus equi

Salvatore Pece, Giuseppe Giuliani, Donato Fumarola, Claudio M. Mastroianni, Miriam Lichtner, Vincenzo Vullo, Salvatore Antonaci, Emilio Jirillo

Research output: Contribution to journalArticlepeer-review

Abstract

The capability of heat-killed Rhodococcus equi organisms to induce in vitro release of tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-8 from normal human mononuclear cells as well as the secretion kinetics of these inflammatory cytokines over a 48 h period were evaluated. Results show that normal human mononuclear cells are efficiently triggered to secrete TNF- α, IL-6 and IL-8 following R. equi stimulation according to a different kinetics. In particular, release of IL-8 was already maximally expressed after 2 h of stimulation, while TNF-α amounts progressively increased in a time-dependent fashion. Finally, IL-6 secretion reached peak levels as soon as 18 h of incubation. Taken together, these data point out that monocyte- derived cytokines may play an important role in the immunological control of R. equi infection in immunocompetent people.

Original languageEnglish
Pages (from-to)277-285
Number of pages9
JournalVeterinary Microbiology
Volume56
Issue number3-4
DOIs
Publication statusPublished - Jun 16 1997

Keywords

  • Interleukin- 8
  • Interleukin-6
  • Polymorphonuclear cells
  • Rhodococcus equi
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

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