In vitro proliferation and in vivo malignancy of cell lines simultaneously derived from a chemically-induced heterogeneous rat mammary tumor

Fausto Tagliaferri, Laura Teodori, Maria Giovanna Valente, Francesco Stipa, Alessandra Cucina, Wolfgang Göhde, Dario Coletti, Piero Alo', Sergio Stipa

Research output: Contribution to journalArticle

Abstract

Identification of clones in primary tumors responsible for proliferation, invasion, and metastasis was carried out. Four different aneuploid established cell lines derived from a ductal infiltrating mammary rat tumor induced by 7,12-dimethylbenz[a]anthracene were studied for proliferative and growth features in vitro and for tumorigenic and metastatic potential in vivo in nude mice. Clones, named RM1, RM2, RM3, and RM4, were characterized by different proliferative activity. Clone RM1 showed the highest proliferative activity by both tritiated thymidine incorporation and S-phase flow cytometry, followed by clone RM4. Conversely, clones RM2 and RM3 showed a lower proliferation rate. Growth-promoting activity, tested on 3T3 Swiss cells, was high in all clones, although RM1 showed significantly lower growth factors - releasing activity. Nude mice tumorigenesis demonstrated a strong tumor induction of line RM1 (100% of the mice after 47 ± 7 d) and a slightly lower tumor induction of line RM4 (70% of the mice after 69 ± 9 d). Line RM3 showed tumor induction in 40% of the mice after 186 ± 16 d. Lines RM2 showed no tumor induction. Metastasis occurred in mice treated with line RM1 only. Therefore, tumorigenesis and metastasis correlate with proliferation but not with the release of growth factors. In conclusion, flow cytometry monitoring of clones from heterogeneous primary tumors proved to be a suitable model for the study of in vivo malignancy and in vitro proliferation.

Original languageEnglish
Pages (from-to)163-166
Number of pages4
JournalIn Vitro Cellular & Developmental Biology - Animal
Volume36
Issue number3
DOIs
Publication statusPublished - Mar 2000

Keywords

  • Breast cancer
  • Flow cytometry
  • Metastatic potential
  • Rat cell lines
  • Tumorigenesis

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Developmental Biology

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