In vitro proliferation and in vivo malignancy of cell lines simultaneously derived from a chemically-induced heterogeneous rat mammary tumor

Fausto Tagliaferri, Laura Teodori, Maria Giovanna Valente, Francesco Stipa, Alessandra Cucina, Wolfgang Göhde, Dario Coletti, Piero Alo', Sergio Stipa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Identification of clones in primary tumors responsible for proliferation, invasion, and metastasis was carried out. Four different aneuploid established cell lines derived from a ductal infiltrating mammary rat tumor induced by 7,12-dimethylbenz[a]anthracene were studied for proliferative and growth features in vitro and for tumorigenic and metastatic potential in vivo in nude mice. Clones, named RM1, RM2, RM3, and RM4, were characterized by different proliferative activity. Clone RM1 showed the highest proliferative activity by both tritiated thymidine incorporation and S-phase flow cytometry, followed by clone RM4. Conversely, clones RM2 and RM3 showed a lower proliferation rate. Growth-promoting activity, tested on 3T3 Swiss cells, was high in all clones, although RM1 showed significantly lower growth factors - releasing activity. Nude mice tumorigenesis demonstrated a strong tumor induction of line RM1 (100% of the mice after 47 ± 7 d) and a slightly lower tumor induction of line RM4 (70% of the mice after 69 ± 9 d). Line RM3 showed tumor induction in 40% of the mice after 186 ± 16 d. Lines RM2 showed no tumor induction. Metastasis occurred in mice treated with line RM1 only. Therefore, tumorigenesis and metastasis correlate with proliferation but not with the release of growth factors. In conclusion, flow cytometry monitoring of clones from heterogeneous primary tumors proved to be a suitable model for the study of in vivo malignancy and in vitro proliferation.

Original languageEnglish
Pages (from-to)163-166
Number of pages4
JournalIn Vitro Cellular & Developmental Biology - Animal
Volume36
Issue number3
DOIs
Publication statusPublished - Mar 2000

Fingerprint

Rats
Tumors
Clone Cells
Cells
Breast Neoplasms
Cell Line
Neoplasms
Flow cytometry
Neoplasm Metastasis
Nude Mice
Intercellular Signaling Peptides and Proteins
Flow Cytometry
Carcinogenesis
Swiss 3T3 Cells
Aneuploidy
In Vitro Techniques
S Phase
Thymidine
Monitoring
Growth

Keywords

  • Breast cancer
  • Flow cytometry
  • Metastatic potential
  • Rat cell lines
  • Tumorigenesis

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Developmental Biology

Cite this

In vitro proliferation and in vivo malignancy of cell lines simultaneously derived from a chemically-induced heterogeneous rat mammary tumor. / Tagliaferri, Fausto; Teodori, Laura; Valente, Maria Giovanna; Stipa, Francesco; Cucina, Alessandra; Göhde, Wolfgang; Coletti, Dario; Alo', Piero; Stipa, Sergio.

In: In Vitro Cellular & Developmental Biology - Animal, Vol. 36, No. 3, 03.2000, p. 163-166.

Research output: Contribution to journalArticle

Tagliaferri, Fausto ; Teodori, Laura ; Valente, Maria Giovanna ; Stipa, Francesco ; Cucina, Alessandra ; Göhde, Wolfgang ; Coletti, Dario ; Alo', Piero ; Stipa, Sergio. / In vitro proliferation and in vivo malignancy of cell lines simultaneously derived from a chemically-induced heterogeneous rat mammary tumor. In: In Vitro Cellular & Developmental Biology - Animal. 2000 ; Vol. 36, No. 3. pp. 163-166.
@article{f11b5f8755af4ce1abff260ae62ae7e2,
title = "In vitro proliferation and in vivo malignancy of cell lines simultaneously derived from a chemically-induced heterogeneous rat mammary tumor",
abstract = "Identification of clones in primary tumors responsible for proliferation, invasion, and metastasis was carried out. Four different aneuploid established cell lines derived from a ductal infiltrating mammary rat tumor induced by 7,12-dimethylbenz[a]anthracene were studied for proliferative and growth features in vitro and for tumorigenic and metastatic potential in vivo in nude mice. Clones, named RM1, RM2, RM3, and RM4, were characterized by different proliferative activity. Clone RM1 showed the highest proliferative activity by both tritiated thymidine incorporation and S-phase flow cytometry, followed by clone RM4. Conversely, clones RM2 and RM3 showed a lower proliferation rate. Growth-promoting activity, tested on 3T3 Swiss cells, was high in all clones, although RM1 showed significantly lower growth factors - releasing activity. Nude mice tumorigenesis demonstrated a strong tumor induction of line RM1 (100{\%} of the mice after 47 ± 7 d) and a slightly lower tumor induction of line RM4 (70{\%} of the mice after 69 ± 9 d). Line RM3 showed tumor induction in 40{\%} of the mice after 186 ± 16 d. Lines RM2 showed no tumor induction. Metastasis occurred in mice treated with line RM1 only. Therefore, tumorigenesis and metastasis correlate with proliferation but not with the release of growth factors. In conclusion, flow cytometry monitoring of clones from heterogeneous primary tumors proved to be a suitable model for the study of in vivo malignancy and in vitro proliferation.",
keywords = "Breast cancer, Flow cytometry, Metastatic potential, Rat cell lines, Tumorigenesis",
author = "Fausto Tagliaferri and Laura Teodori and Valente, {Maria Giovanna} and Francesco Stipa and Alessandra Cucina and Wolfgang G{\"o}hde and Dario Coletti and Piero Alo' and Sergio Stipa",
year = "2000",
month = "3",
doi = "10.1290/1071-2690(2000)036<0163:IVPAIV>2.3.CO;2",
language = "English",
volume = "36",
pages = "163--166",
journal = "In Vitro Cellular and Developmental Biology - Animal",
issn = "1071-2690",
publisher = "Springer New York",
number = "3",

}

TY - JOUR

T1 - In vitro proliferation and in vivo malignancy of cell lines simultaneously derived from a chemically-induced heterogeneous rat mammary tumor

AU - Tagliaferri, Fausto

AU - Teodori, Laura

AU - Valente, Maria Giovanna

AU - Stipa, Francesco

AU - Cucina, Alessandra

AU - Göhde, Wolfgang

AU - Coletti, Dario

AU - Alo', Piero

AU - Stipa, Sergio

PY - 2000/3

Y1 - 2000/3

N2 - Identification of clones in primary tumors responsible for proliferation, invasion, and metastasis was carried out. Four different aneuploid established cell lines derived from a ductal infiltrating mammary rat tumor induced by 7,12-dimethylbenz[a]anthracene were studied for proliferative and growth features in vitro and for tumorigenic and metastatic potential in vivo in nude mice. Clones, named RM1, RM2, RM3, and RM4, were characterized by different proliferative activity. Clone RM1 showed the highest proliferative activity by both tritiated thymidine incorporation and S-phase flow cytometry, followed by clone RM4. Conversely, clones RM2 and RM3 showed a lower proliferation rate. Growth-promoting activity, tested on 3T3 Swiss cells, was high in all clones, although RM1 showed significantly lower growth factors - releasing activity. Nude mice tumorigenesis demonstrated a strong tumor induction of line RM1 (100% of the mice after 47 ± 7 d) and a slightly lower tumor induction of line RM4 (70% of the mice after 69 ± 9 d). Line RM3 showed tumor induction in 40% of the mice after 186 ± 16 d. Lines RM2 showed no tumor induction. Metastasis occurred in mice treated with line RM1 only. Therefore, tumorigenesis and metastasis correlate with proliferation but not with the release of growth factors. In conclusion, flow cytometry monitoring of clones from heterogeneous primary tumors proved to be a suitable model for the study of in vivo malignancy and in vitro proliferation.

AB - Identification of clones in primary tumors responsible for proliferation, invasion, and metastasis was carried out. Four different aneuploid established cell lines derived from a ductal infiltrating mammary rat tumor induced by 7,12-dimethylbenz[a]anthracene were studied for proliferative and growth features in vitro and for tumorigenic and metastatic potential in vivo in nude mice. Clones, named RM1, RM2, RM3, and RM4, were characterized by different proliferative activity. Clone RM1 showed the highest proliferative activity by both tritiated thymidine incorporation and S-phase flow cytometry, followed by clone RM4. Conversely, clones RM2 and RM3 showed a lower proliferation rate. Growth-promoting activity, tested on 3T3 Swiss cells, was high in all clones, although RM1 showed significantly lower growth factors - releasing activity. Nude mice tumorigenesis demonstrated a strong tumor induction of line RM1 (100% of the mice after 47 ± 7 d) and a slightly lower tumor induction of line RM4 (70% of the mice after 69 ± 9 d). Line RM3 showed tumor induction in 40% of the mice after 186 ± 16 d. Lines RM2 showed no tumor induction. Metastasis occurred in mice treated with line RM1 only. Therefore, tumorigenesis and metastasis correlate with proliferation but not with the release of growth factors. In conclusion, flow cytometry monitoring of clones from heterogeneous primary tumors proved to be a suitable model for the study of in vivo malignancy and in vitro proliferation.

KW - Breast cancer

KW - Flow cytometry

KW - Metastatic potential

KW - Rat cell lines

KW - Tumorigenesis

UR - http://www.scopus.com/inward/record.url?scp=0034078441&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034078441&partnerID=8YFLogxK

U2 - 10.1290/1071-2690(2000)036<0163:IVPAIV>2.3.CO;2

DO - 10.1290/1071-2690(2000)036<0163:IVPAIV>2.3.CO;2

M3 - Article

C2 - 10777055

AN - SCOPUS:0034078441

VL - 36

SP - 163

EP - 166

JO - In Vitro Cellular and Developmental Biology - Animal

JF - In Vitro Cellular and Developmental Biology - Animal

SN - 1071-2690

IS - 3

ER -